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자료유형
학술저널
저자정보
Lincon Rafael da Silva (Embrapa Genetic Resources and Biotechnology) Leonardo Luís de Barros Rodrigues (College Union Pioneer of Social Integration) Amanda Silva Botelho (University of Brasília) Bruna Sartório de Castro (University of Brasília) Paulo Henrique Pereira Costa Muniz (State University of Goiás) Maria Carolina Blassioli Moraes (Embrapa Genetic Resources and Biotechnology) Sueli Corrêa Marques de Mello (Embrapa Genetic Resources and Biotechnology)
저널정보
한국식물병리학회 The Plant Pathology Journal The Plant Pathology Journal 제39권 제1호
발행연도
2023.2
수록면
39 - 51 (13page)

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Common bean (Phaseolus vulgaris L.) is one of the most important crops in human food production. The occurrence of diseases, such as white mold, caused by Sclerotinia sclerotiorum can limit the production of this legume. The use of Trichoderma has become an important strategy in the suppression of this disease. The aim of the present study was to evaluate the effect of volatile organic compounds (VOCs) emitted by Trichoderma azevedoi CEN1241 in five different growth periods on the severity of white mold in common bean. The in vitro assays were carried out in double-plate and split-plate, and the in vivo assays, through the exposure of the mycelia of S. sclerotiorum to the VOCs of T. azevedoi CEN1241 and subsequent inoculation in bean plants. Chemical analysis by gas chromatography coupled to mass spectrometry detected 37 VOCs produced by T. azevedoi CEN1241, covering six major chemical classes. The profile of VOCs produced by T. azevedoi CEN1241 varied according to colony age and was shown to be related to the ability of the biocontrol agent to suppress S. sclerotiorum. T. azevedoi CEN1241 VOCs reduced the size of S. sclerotiorum lesions on bean fragments in vitro and reduced disease severity in a greenhouse. This study demonstrated in a more applied way that the mechanism of antibiosis through the production of volatile compounds exerted by Trichoderma can complement other mechanisms, such as parasitism and competition, thus contributing to a better efficiency in the control of white mold in bean plants.

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Materials and Methods
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Discussion
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