지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2022.8
- 수록면
- 417 - 422 (6page)
이용수
초록· 키워드
오류제보하기
Apple stem grooving virus (ASGV) is a destructive viral pathogen of pome fruit trees that causes significant losses to fruit production worldwide. Obtaining ASGV-free propagation materials is essential to reduce economic losses, and accurate and sensitive detection methods to screen ASGV-free plantlets during in vitro propagation are urgently necessary. In this study, ASGV was sensitively and accurately quantified from in vitro propagated apple plantlets using a reverse transcription droplet digital polymerase chain reaction (RT-ddPCR) assay. The optimized RT-ddPCR assay was specific to other apple viruses, and was at least 10-times more sensitive than RT-real-time quantitative PCR assay. Furthermore, the optimized RT-ddPCR assay was validated for the detection and quantification of ASGV using micropropagated apple plantlet samples. This RT-ddPCR assay can be utilized for the accurate quantitative detection of ASGV infection in ASGV-free certification programs, and can thus contribute to the production of ASGV-free apple trees.
목차
등록된 정보가 없습니다.
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
A Reliable Reverse Transcription Loop-Mediated Isothermal Amplification Assay for Detecting Apple stem grooving virus in Pear
Research in Plant Disease(식물병연구)
2022 .06
-
Rapid and Specific Detection of Apple stem grooving virus by Reverse Transcription-recombinase Polymerase Amplification
The Plant Pathology Journal
2018 .12
-
Phylogenetic and Recombination Analysis of Apple Stem Grooving Virus Isolates from Pears in Korea
Research in Plant Disease(식물병연구)
2023 .06
-
Detection of Rift Valley Fever Virus Using RT-qPCR and RT-ddPCR
한국미생물학회 학술대회논문집
2022 .10
-
Comparison of Digital PCR and Quantitative PCR with Various SARS-CoV-2 Primer-Probe Sets
Journal of Microbiology and Biotechnology
2021 .01
-
Survey of Major Viruses in Commercial Nursery Trees of Major Pear Cultivars in Korea
Research in Plant Disease(식물병연구)
2019 .03
-
Reverse Transcription Droplet Digital PCR을 활용한 Tomato Spotted Wilt Virus 검출 및 정량
Research in Plant Disease(식물병연구)
2021 .09
-
국내 주요지역의 사과 바이러스 및 바이로이드 5종의 발생 현황
Research in Plant Disease(식물병연구)
2020 .06
-
국내 유통 주요 사과나무 묘목의 바이러스 감염 실태
Research in Plant Disease(식물병연구)
2017 .12
-
Identification of Plant Viruses Infecting Pear Using RNA Sequencing
The Plant Pathology Journal
2021 .06
-
Evaluation of Various Real-Time Reverse Transcription Quantitative PCR Assays for Norovirus Detection
Journal of Microbiology and Biotechnology
2017 .01
-
Development of a One-Step Multiplex Reverse Transcription- Quantitative Polymerase Chain Reaction Assay for the Simultaneous Detection of Four Lily Viruses
Research in Plant Disease(식물병연구)
2024 .12
-
Detection of Apple Scar Skin Viroid by Reverse Transcription Recombinase Polymerase Amplification Assay
Research in Plant Disease(식물병연구)
2021 .06
-
Development of a Rapid Detection Method for Potato virus X by Reverse Transcription Loop-Mediated Isothermal Amplification
The Plant Pathology Journal
2015 .09
-
Deep Sequencing Analysis of Apple Infecting Viruses in Korea
The Plant Pathology Journal
2016 .10
-
Development and Application of Reverse Transcription Nanoplate-Based Digital PCR Assay for Sensitive and Accurate Detection of Rice Black-Streaked Dwarf Virus in Cereal Crops
The Plant Pathology Journal
2024 .08
-
Rapid and Sensitive Detection of Lettuce Necrotic Yellows Virus and Cucumber Mosaic Virus Infecting Lettuce (Lactuca sativa L.) by Reverse Transcription Loop-Mediated Isothermal Amplification
The Plant Pathology Journal
2020 .02
-
Development of a Multiplex Reverse Transcription-Polymerase Chain Reaction Assay for the Simultaneous Detection of Three Viruses in Leguminous Plants
Research in Plant Disease(식물병연구)
2018 .12
-
Application of Rapid and Reliable Detection of Cymbidium Mosaic Virus by Reverse Transcription Recombinase Polymerase Amplification Combined with Lateral Flow Immunoassay
The Plant Pathology Journal
2022 .12
-
Reverse Transcription Recombinase Polymerase Amplification Assay for Rapid and Sensitive Detection of Barley Yellow Dwarf Virus in Oat
The Plant Pathology Journal
2020 .10
이 논문의 저자 정보
최근 본 자료
댓글(0)
0
UCI(KEPA) : I410-ECN-0101-2023-481-001544371