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논문 기본 정보

자료유형
학술저널
저자정보
Ainur Ainiah Azman Husni (Universiti Putra Malaysia) Siti Izera Ismail (Universiti Putra Malaysia) Noraini Md. Jaafar (Universiti Putra Malaysia) Dzarifah Zulperi (Universiti Putra Malaysia)
저널정보
한국식물병리학회 The Plant Pathology Journal The Plant Pathology Journal 제37권 제3호
발행연도
2021.6
수록면
243 - 257 (15page)

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Bacillus pumilus is the causal agent of trunk bulges disease affecting rubber and rubberwood quality and yield production. In this study, B. pumilus and other closely related species were included in B. pumilus group, as they shared over 99.5% similarity from 16S rRNA analysis. Multilocus sequence analysis (MLSA) of five housekeeping genes and repetitive elements-based polymerase chain reaction (rep-PCR) using REP, ERIC, and BOX primers conducted to analyze the diversity and systematic relationships of 20 isolates of B. pumilus group from four rubber tree plantations in Peninsular Malaysia (Serdang, Tanah Merah, Baling, and Rawang). Multi rep-PCR results revealed the genetic profiling among the B. pumilus group isolates, while MLSA results showed 98-100% similarity across the 20 isolates of B. pumilus group species. These 20 isolates, formerly established as B. pumilus, were found not to be grouped with B. pumilus. However, being distributed within distinctive groups of the B. pumilus group comprising of two clusters, A and B. Cluster A contained of 17 isolates close to B. altitudinis, whereas Cluster B consisted of three isolates attributed to B. safensis. This is the first MLSA and rep-PCR study on B. pumilus group, which provides an in-depth understanding of the diversity of these rubber-pathogenic isolates in Malaysia.

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Materials and Methods
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