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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2023.6
- 수록면
- 72 - 79 (8page)
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MRSA is Staphylococcus aureus resistant to β-lactam antibiotics, and is a worldwide infectious disease. Even with the discovery of new antibiotics, resistance develops rapidly, so new alternatives are needed. Jakyakgamcho-tang (JGT) is a combination of Jakyak and Gamcho, and has been mainly used as an antispasmodic and analgesic in oriental medicine. This study was conducted to find out whether there is an effect on MRSA in relation to the anti-inflammatory effect of JGT and the antibacterial effect of Jakyak and Gamcho found in previous studies. In this study, in order to investigate the antibacterial activity of JGT and the combined effect of existing antibiotics, after extracting JGT with 70% EtoH, the disc diffusion method, minimum inhibitory concentration (MIC), drug combination effect (FICI), and time-kill analysis (Time-kill assay), metabolic inhibition, Western blot and qRT-PCR analysis were used to confirm the antibacterial activity mechanism of MRSA of JGT. As a result of the experiment, all of MRSA showed antibacterial activity in JGT’s disc diffusion method, and the MIC was 250 -1000 ㎍/mL. When existing antibiotics and JGT were combined with drugs, most had synergy or partial synergy. In addition, it was confirmed that the degree of bacterial growth was suppressed over time when simultaneous administration for 24 hours. JGT showed a synergistic effect when administered together with the ATPase-inhibitor DCCD, suggesting that it affected the inhibition of ATPase. As a result of observing the expression of PBP2a, and hla protein in the JGT-treated group and the untreated control group through wstern blot, it was confirmed that the protein expression of the JGT-treated group was significantly suppressed, and the expression levels of mecA, mecR1 and hla genes were also suppressed during JGT treatment. was observed by qRT-PCR. Combining the results of the experiment, it can be seen that JGT has antibacterial activity in MRSA, and when combined with existing antibiotics, the effect was increased compared to treatment with the drug alone. This suggests that JGT can be an alternative to treatment for antibiotic resistance of MRSA.
목차
Abstract
재료 및 방법
결과
고찰
결론
인용문헌
참고문헌 (17)
참고문헌 신청
Lakhundi, S. and Zhang, K. (2018) Methicillin-resistant Staphylococcus aureus: Molecular characterization, evolution, and epidemiology. Clin. Microbiol. Rev. 31: e00020-18.
Gould, D. and Chamberlaine, A. (1995) Staphylococcus aureus: a review of the literature. J. Clin. Nurs. 4: 5-12. https://doi.org/10.1111/j.1365-2702.1995.tb00004.x
Loffler, B. and Tuchscherr, L. (2021) Staphylococcus aureus toxins: Promoter or handicap during infection? Toxins (Basel) 13: 287.
Zheng, D., Chen, Y., Wan, S., Jiang, J., Chen, S., Zheng, C., Zhou, H., Xu, G., Zhang, H. and Xu, H. (2021) Polycyclic polyprenylated acylphloroglucinol congeners from Garcinia yunnanensis Hu with inhibitory effect on α-hemolysin production in Staphylococcus aureus. Bioorg. Chem. 114: 105074.
Li, Q. Q., Luo, J., Liu, X. Q., Kang, O. H. and Kwon, D. Y. (2021) Eleutheroside K isolated from Acanthopanax henryi (Oliv.) Harms inhibits the expression of virulence-related exoproteins in methicillin-resistant Staphylococcus aureus. Curr. Microbiol. 78: 3980-3988. https://doi.org/10.1007/s00284-021-02631-5
Gould, D. and Chamberlaine, A. (1995) Staphylococcus aureus: a review of the literature. J. Clin. Nurs. 4: 5-12. https://doi.org/10.1111/j.1365-2702.1995.tb00004.x
Loffler, B. and Tuchscherr, L. (2021) Staphylococcus aureus toxins: Promoter or handicap during infection? Toxins (Basel) 13: 287.
Zheng, D., Chen, Y., Wan, S., Jiang, J., Chen, S., Zheng, C., Zhou, H., Xu, G., Zhang, H. and Xu, H. (2021) Polycyclic polyprenylated acylphloroglucinol congeners from Garcinia yunnanensis Hu with inhibitory effect on α-hemolysin production in Staphylococcus aureus. Bioorg. Chem. 114: 105074.
Li, Q. Q., Luo, J., Liu, X. Q., Kang, O. H. and Kwon, D. Y. (2021) Eleutheroside K isolated from Acanthopanax henryi (Oliv.) Harms inhibits the expression of virulence-related exoproteins in methicillin-resistant Staphylococcus aureus. Curr. Microbiol. 78: 3980-3988. https://doi.org/10.1007/s00284-021-02631-5
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