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논문 기본 정보

자료유형
학술저널
저자정보
Kim Ju Hee (Chonnam National University) Kim Mi Young (Chonnam National University) Lee Jae Hyun (Chonnam National University) Kim Jang Uk (Department of Herbal Crop Research NIHHS RDA Eumseong) 이옥란 (전남대학교)
저널정보
한국식물생명공학회 Plant Biotechnology Reports Plant Biotechnology Reports 제17권 제3호
발행연도
2023.6
수록면
331 - 339 (9page)
DOI
10.1007/s11816-023-00839-4

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In vitro regenerated ginseng plants from embryos have been successfully ex vitro acclimatized and transplanted to soil as reported in previous studies. However, resprouting of ex vitro acclimatized ginseng in the following year has not been achieved. In this study, we optimized plant regeneration protocol through the somatic embryo culture and acclimatization steps in the soil for Korean ginseng. Primary somatic embryos were induced from the cut cotyledons in hormone-free MS medium with 3% sucrose after 8 weeks. The secondary somatic embryos were induced from transplanted primary embryos in hormone-supplemented MS containing 1 mg/L 2,4-D and 0.5 mg/L BAP for 10 weeks. Two weeks more cultivation in the same medium generated shoots, and shoot elongation and the formation of all above-ground parts of the shoots was achieved in hormone-free MS medium over 6 weeks. Roots were formed in 1/2 MS medium with 1% sucrose and 0.3% of activated charcoal after 12 weeks. Overall, plant generation, from the cotyledon of the zygote to the transplantable plantlet, was about 53% efficient (33 plantlet/61 secondary embryos) and took about 38 weeks. The acclimatization of transplanted plantlets in a soil mixture was 82% efficient. Buds on taproots of adapted plants developed a dormant tendency, and the dormancy of these buds was broken during cold treatment at 4 °C for 3 to 4 months. The system we developed can be effectively utilized for the mass production of Korean ginseng through somatic embryo culture.

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