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논문 기본 정보

자료유형
학술저널
저자정보
Moon Young-Gun (Department of aquatic Biomedical Sciences, Jeju National University, Jeju 63243, Republic of Korea) Boo Moon-Soo (Fishing Coporation CR Co., Ltd., Jeju 63333, Republic of Korea) Lee Chi-Hoon (Fishing Coporation CR Co., Ltd., Jeju 63333, Republic of Korea) Park Jin-Kuk (Department of aquatic Biomedical Sciences, Jeju National University, Jeju 63243, Republic of Korea) Heo Moon-Soo (Department of aquatic Biomedical Sciences, Jeju National University, Jeju 63243, Republic of Korea)
저널정보
한국미생물생명공학회 한국미생물·생명공학회지 한국미생물·생명공학회지 제52권 제1호
발행연도
2024.3
수록면
1 - 14 (14page)
DOI
10.48022/mbl.2309.09001

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초록· 키워드

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This study focused on isolating and identifying strains from the gut of Epinephelus akaara cultivated in aquaculture facilities on Jeju Island. The aim was to evaluate the potential of utilizing these strains as probiotics for industrial applications. A total of 129 strains were isolated from the gut of E. akaara and screened based on their ability to create a clear zone of 10 mm or more in a preliminary antimicrobial activity test. Twelve strains were selected for further analysis, including bile resistance, acid tolerance at different pH levels, antioxidant activity, antibiotic susceptibility, and biochemical characteristics using the API kit. Through these characteristic experiments, eight strains (G1, G3, G15, G21, B1, B2, B3, B5) were identified as having potential as probiotics. Among these, the B group strains (B1, B2, B3, B5) exhibited significantly higher activity compared to the G group strains (G1, G3, G15, G21). Based on the phylogenetic analysis of the 16S rRNA gene sequences of the selected microorganisms, the strains were named as follows: B1 strain as Lactobacillus paracasei B1, B2 strain as Lactococcus lactis B2, B3 strain as Lactobacillus plantarum B3, B5 strain as Lactococcus lactis subsp. hordniae B5, G1 strain as Bacillus licheniformis G1, G3 strain as Bacillus velezensis G3, G15 strain as Brevibacterium frigoritolerans G15, and G21 strain as Bacillus pumilus G21.

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