지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2024.2
- 수록면
- 40 - 47 (8page)
이용수
초록· 키워드
오류제보하기
Garlic can be infected by a variety of viruses, but mixed infections with leek yellow stripe virus, onion yellow dwarf virus, and allexiviruses are the most damaging, so an easy, inexpensive on-site method to simultaneously detect at least these three viruses with a certain degree of accuracy is needed to produce virus-free plants. The most common laboratory method for diagnosis is multiplex reverse transcription polymerase chain reaction (RT-PCR). However, allexiviruses are highly diverse even within the same species, making it difficult to design universal PCR primers for all garlic-growing regions in the world. To solve this problem, we developed an inexpensive on-site detection system for the three garlic viruses that uses a commercial mobile PCR device and a compact electrophoresis system with a blue light. In this system, virus-specific bands generated by electrophoresis can be identified by eye in real time because the PCR products are labeled with a fluorescent dye, FITC. Because the electrophoresis step might eventually be replaced with a lateral flow assay (LFA), we also demonstrated that a uniplex LFA can be used for virus detection; however, multiplexing and a significant cost reduction are needed before it can be used for on-site detection.
목차
Materials and Methods
Results
Discussion
References
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Development of a Multiplex Polymerase Chain Reaction Assay for Detecting Five Previously Unreported Papaya Viruses for Quarantine Purposes in Korea
Research in Plant Disease(식물병연구)
2024 .09
-
Development of a Multiplex Reverse Transcription-Polymerase Chain Reaction Assay for the Simultaneous Detection of Three Viruses in Leguminous Plants
Research in Plant Disease(식물병연구)
2018 .12
-
Development of a Multiplex RT-PCR for the Simultaneous Detection of Three Viruses in Cherry Plants
Research in Plant Disease(식물병연구)
2018 .09
-
Development of Multiplex RT-PCR for Simultaneous Detection of Garlic Viruses and the Incidence of Garlic Viral Disease in Garlic Genetic Resources
The Plant Pathology Journal
2015 .03
-
Simultaneous Detection of Four Foodborne Viruses in Food Samples Using a One-Step Multiplex Reverse Transcription PCR
Journal of Microbiology and Biotechnology
2018 .01
-
Post-pandemic influenza A (H1N1) virus detection by real-time PCR and virus isolation
미생물학회지
2019 .03
-
Comprehensive Metatranscriptomic Analysis of Plant Viruses in Imported Frozen Cherries and Blueberries
The Plant Pathology Journal
2024 .08
-
Occurrence and Multiplex PCR Detection of Citrus Yellow Vein Clearing Virus in Korea
The Plant Pathology Journal
2024 .04
-
The Plant Cellular Systems for Plant Virus Movement
The Plant Pathology Journal
2017 .06
-
Pathogenesis of Oncoviruses: A Systemic Review
한국미생물·생명공학회지
2023 .06
-
Incidence of Viral Diseases and Occurrence of Three Unreported Viruses in Yams in Korea
Research in Plant Disease(식물병연구)
2017 .03
-
Computation of Refractive Indices of Corona Viruses through Reverse Calculation
Current Optics and Photonics
2020 .12
-
Identification of Plant Viruses Infecting Pear Using RNA Sequencing
The Plant Pathology Journal
2021 .06
-
The Detection of Plant Viruses in Korean Ginseng (Panax ginseng) through RNA Sequencing
The Plant Pathology Journal
2020 .12
-
Development of a Multiplex PCR for Simultaneous Detection of Blueberry Red Ringspot Virus and Blueberry Scorch Virus Including an Internal Control
Research in Plant Disease(식물병연구)
2023 .03
-
Structure and Function of the Influenza A Virus Non-Structural Protein 1
Journal of Microbiology and Biotechnology
2019 .01
-
Rapid and Visual Detection of Barley Yellow Dwarf Virus by Reverse Transcription Recombinase Polymerase Amplification with Lateral Flow Strips
The Plant Pathology Journal
2022 .04
-
Detection of Plant Pathogenic Viruses in Commercial Gochujang (Fermented Red Pepper Paste) from Korea
The Plant Pathology Journal
2020 .10
-
Emergence of Viruses, Mechanisms and Recent Examples
한국미생물학회 학술대회논문집
2015 .04
-
Correlation between Infection with Multiple Respiratory Viruses and Length of Hospital Stay in Patients from Cheonan, Korea
대한임상검사과학회지
2017 .01
최근 본 자료
댓글(0)
0