지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2024.6
- 수록면
- 59 - 68 (10page)
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초록· 키워드
오류제보하기
Heterogeneous immunoassays using CdSe/ZnS quantum dots (QDs) to label anti-progesterone antibody (P₄Ab) were developed for the determination of progesterone (P₄) in the wells of a 96-well microtiter plate. P₄Ab was conjugated to hydrophilic CdSe/ZnS QDs via ethyl-3-(dimethylaminopropyl) carbodiimide (EDC)-N-hydroxysuccinimide (NHS) chemistry (QDs-P₄Ab conjugates). The QDs-P₄Ab conjugates were employed as a second antibody in a sandwich immunoassay or immobilized onto the 3-aminopropyltrimethoxysilane (APTMS) sol-gelmembrane in a direct binding assay. The sandwich immunoassay had a detection limit of 553.9 pg/ml and a sensitivity of 18,251.96 pg/ml with a linear range of 2,184.6 -117,082 pg/ml, while the direct binding immunoassay had a linear range of 28.95 -26,607.7 pg/ml with a detection limit of 3.32 pg/ml and a sensitivity of 987.24 pg/ml. These fluorescence immunoassays have been successfully applied for the determination of P₄ in real human serum, and the resultswere well correlatedwith those of a certified radioimmunoassay (RIA)method.
목차
Abstract
1. INTRODUCTION
2. MATERIALS AND METHODS
3. RESULTS AND DISCUSSION
4. CONCLUSIONS
REFERENCES
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