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논문 기본 정보

자료유형
학술저널
저자정보
Kim Ki Tae (Southeast Sea Fisheries Research Institute, National Institute of Fisheries Science) Kim Mi Ae (Gangneung-Wonju National University) Kim Woo Jin (Biotechnology Research Division, National Institute of Fisheries Science) Jung Min Min (Subtropical Fisheries Research Institute, National Institute of Fisheries Science) Kim Dong Hwi (East Sea Fisheries Research Institute, National Institute of Fisheries Science) Sohn Young Chang (Gangneung-Wonju National University)
저널정보
한국유전학회 Genes & Genomics Genes and Genomics Vol.46 No.8
발행연도
2024.8
수록면
955 - 966 (12page)
DOI
10.1007/s13258-024-01537-3

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Background The genes involved in cephalopod development and their association with hatching and survival during early life stages have been extensively studied. However, few studies have investigated the paralarvae transcriptome of the East Asian common octopus (Octopus sinen sis). Objective This study aimed to identify the genes related to embryonic development and hatching in O. sinensis using RNA sequencing (RNA-seq) and verify the genes most relevant to different embryonic stages. Methods RNA samples from hatched and 25 days post-hatching (dph) O. sinensis paralarvae were used to construct cDNA libraries. Clean reads from individual samples were aligned to the reference O. sinensis database to identify the differentially expressed genes (DEGs) between the 0- and 25-dph paralarvae libraries. Real-time quantitative polymerase chain reaction (RT-qPCR) was used to supplement the RNA-seq data for embryogenic developmental stages. Results A total of 12,597 transcripts were annotated and 5,468 DEGs were identified between the 0- and 25-dph O. sinensis paralarvae, including 2,715 upregulated and 2,753 downregulated transcripts in the 25-dph paralarvae. Several key DEGs were related to transmembrane transport, lipid biosynthesis, monooxygenase activity, lipid transport, neuropeptide signaling, transcription regulation, and protein-cysteine S-palmitoyltransferase activity during the post-hatching development of O. sinensis paralarvae. RT-qPCR analysis further revealed that SLC5A3A, ABCC12, and NPC1 transcripts in 20 and/or 30 days post-fertilization (dpf) embryos were significantly higher (p < 0.05) than those in 10-dpf embryos. Conclusion Transcriptome profiles provide molecular targets to understand the embryonic development, hatching, and survival of O. sinensis paralarvae, and enhance octopus production.

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