지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2024.3
- 수록면
- 62 - 68 (7page)
이용수
초록· 키워드
오류제보하기
The Suspension Trapping (S-Trap) method has been a prominent sample preparation technique since its introduction in 2014. Its capacity to induce protein aggregation using organic solvents has significantly improved protein purification and facilitated peptide identification. However, its full potential for automation has been limited by the lack of a suitable liquid handling system until recently. In this study, we aimed to enhance the automation of S-Trap sample preparation by optimizing the STrap digestion process, incorporating triethylammonium bicarbonate (TEAB) and CaCl₂. The utilization of TEAB buffer conditions in this innovative process led to a noteworthy 12% improvement in protein identification. Additionally, through careful observation of various incubation conditions, we streamlined the entire sample preparation workflow into a concise 4 hours timeline, covering reduction, alkylation, and trypsin incubation stages. This refined and expedited automated S-Trap digestion process not only showcased exceptional time efficiency but also improved trypsin digestion, resulting in increased protein identification.
목차
Abstract
Introduction
Materials and methods
Results and Discussion
Conclusions
References
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Optimum Radius Size between Cylindrical Ion Trap and Quadrupole Ion Trap
Mass Spectrometry Letters
2015 .09
-
Effects of Heat Shock Treatment on Enzymatic Proteolysis for LC-MS/MS Quantitative Proteome Analysis
Mass Spectrometry Letters
2016 .03
-
Sequential purge and trap technique for sampling of geosmin and 2-MIB in aqueous samples using needle trap device (NTD)
한국분석과학회 학술대회
2016 .11
-
Analysis of Trap Distribution of MOSFET using Charge Pumping Method
한국진공학회 학술발표회초록집
2017 .08
-
Effect of centrifugation on tryptic protein digestion
분석과학
2017 .04
-
Off-Current Analysis with Temperature-Dependent Subthreshold Conduction in SiTe Based Amorphous Chalcogenides for Ovonic Threshold Switching Selector Application
Applied Science and Convergence Technology
2024 .07
-
Applications of Stochastic Process in the Quadrupole Ion traps
Mass Spectrometry Letters
2015 .12
-
Charge pumping method를 이용한 MOSFET소자의 Trap분포 연구
한국진공학회 학술발표회초록집
2015 .08
-
Study of CO2+(CO2)n Cluster in a Paul Ion Trap
Mass Spectrometry Letters
2019 .03
-
빙하코어를 이용한 과거 대기 산화이질소 농도 분석법 개발
대한지질학회 학술대회
2015 .10
-
Investigation of Ne and He Buffer Gases Cooled Ar+ Ion Clouds in a Paul Ion Trap
Mass Spectrometry Letters
2015 .12
-
Quantum vacuum standards : making practical tools out of cold trapped atoms
한국진공학회 학술발표회초록집
2016 .08
-
개미류 신속발견을 위한 다단협관유도트랩 개발
환경생물
2022 .09
-
TCharge trap 층에 금속 공간층 삽입에 따른 charge trap flash 메모리 소자의 전기적인 특성
한국진공학회 학술발표회초록집
2015 .08
-
Analysis on interface trap density of MOS capacitor with Al-doped HfO₂ insulation layer
한국진공학회 학술발표회초록집
2020 .02
-
Effect of centrifugation on tryptic digestion of proteins
한국분석과학회 학술대회
2016 .11
-
Nonvolatile charge trap memory device with In-Ga-Zn-O active channel layer using Microwave Irradiation
한국진공학회 학술발표회초록집
2015 .02
-
시설딸기 농장에서 황색점착트랩 설치각도에 따른 주요 해충의 유살특성 예비연구
한국응용곤충학회지
2019 .01
-
Effects of digestion temperatures and loading amounts on methane production from anaerobic digestion with crop residues
Carbon letters
2015 .01
-
Trap suppression in ferroelectric-polymer vdW synaptic device through h-BN layer
한국진공학회 학술발표회초록집
2019 .08
이 논문의 저자 정보
최근 본 자료
댓글(0)
0