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논문 기본 정보

저자정보
(Korea Food Research Institute) (Korea Food Research Institute)
저널정보
한국식품과학회 한국식품과학회지 한국식품과학회지 제57권 제2호
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    초록·키워드

    Freeze-drying is widely used for microbial preservation. However, maintaining viability without cryoprotectants remains a challenge, particularly for Saccharomyces cerevisiae. This study aimed to optimize the freeze-drying parameters without using protective additives. Key variables, including pre-treatment temperature and duration, initial and final shelf temperatures, and initial cell concentration, were systematically evaluated. The highest viability was achieved with pretreatment at 4℃ for 24 h, an initial shelf temperature of -0.5℃, a final shelf temperature of 40℃, and an initial cell concentration of log 9 cells/mL. Under these conditions, the viable cell count reached 1.0 X10<sup>10</sup> cells/g with a viability yield of 21.6%. These results show that appropriate adjustment of freeze-drying conditions can preserve yeast viability without the need for cryoprotectants. This approach offers a cost-effective alternative for industrial applications and provides a foundation for future improvements through strain-specific optimization or selective use of protective agents.

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