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DBpia AI
AI 에이전트
AI 아이디어
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Citeasy
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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2026.6
- 수록면
- 398 - 416 (19page)
이용수
초록· 키워드
BACKGROUND/OBJECTIVES: Diabetic nephropathy (DN) is one of the most frequent and serious complications of diabetes mellitus. A low-protein diet supplemented with an α-ketoacid diet (LPD-KA) is a crucial intervention for individuals with chronic kidney disease. Nevertheless, the effects and mechanism of LPD-KA in DN remain unclear.
MATERIALS/METHODS: Db/db mice were randomly assigned into three groups: normal protein diet (NPD), low-protein diet (LPD), and LPD-KA groups, with db/m mice fed with NPD as the Control (Cont) group. PAS and Masson staining were performed after detecting the weight, blood glucose, and renal biochemical indicators. 16S rRNA sequencing and Urine metabolomics were conducted.
RESULTS: LPD-KA for DN mice improved the renal tissue damage and fibrosis, with lower body and kidney weights, blood glucose, urinary glucose, serum creatinine, blood urea nitrogen, urinary albumin/creatinine ratio, 24-h urine microalbumin, and glycosylated hemoglobin A1c levels. Compared to the NPD group, LPD-KA increased relative abundance of Firmicutes/ Bacteroides, f_Lachnospiraceae, g_Akkermansia, g_Clostridium, g_Staphylococcus, g_Enterococcus, and f_Enterococcaceae. Moreover, LPD-KA altered urinary metabolomics, including up-regulation of nephroprotective differentially expressed metabolites (DEMs) L-histidinol and gluconolactone, and down-regulation of nephrotoxic DEMs 2-ketobutyric acid, dihydrocortisol, citramalic acid, and L-erythrulose, which were enriched in the protein digestion and absorption and tyrosine metabolism pathway. Spearman’s correlation analysis revealed strong correlation between the crucial genus flora ( Lactobacillus, Akkermansia, Bacteroides, Adlercreutzia, Rikenella, and Clostridium) and the above metabolites.
CONCLUSION: LPD-KA modulated the intestinal flora and urinary metabolism to attenuate DN progression, with Lactobacillus, Akkermansia, Bacteroides, L-histidinol, gluconolactone, 2-ketobutyric acid, and dihydrocortisol as potential biomarkers, providing new insights into the clinical application of LPD-KA for DN treatment.
상세정보 수정요청해당 페이지 내 제목·저자·목차·페이지MATERIALS/METHODS: Db/db mice were randomly assigned into three groups: normal protein diet (NPD), low-protein diet (LPD), and LPD-KA groups, with db/m mice fed with NPD as the Control (Cont) group. PAS and Masson staining were performed after detecting the weight, blood glucose, and renal biochemical indicators. 16S rRNA sequencing and Urine metabolomics were conducted.
RESULTS: LPD-KA for DN mice improved the renal tissue damage and fibrosis, with lower body and kidney weights, blood glucose, urinary glucose, serum creatinine, blood urea nitrogen, urinary albumin/creatinine ratio, 24-h urine microalbumin, and glycosylated hemoglobin A1c levels. Compared to the NPD group, LPD-KA increased relative abundance of Firmicutes/ Bacteroides, f_Lachnospiraceae, g_Akkermansia, g_Clostridium, g_Staphylococcus, g_Enterococcus, and f_Enterococcaceae. Moreover, LPD-KA altered urinary metabolomics, including up-regulation of nephroprotective differentially expressed metabolites (DEMs) L-histidinol and gluconolactone, and down-regulation of nephrotoxic DEMs 2-ketobutyric acid, dihydrocortisol, citramalic acid, and L-erythrulose, which were enriched in the protein digestion and absorption and tyrosine metabolism pathway. Spearman’s correlation analysis revealed strong correlation between the crucial genus flora ( Lactobacillus, Akkermansia, Bacteroides, Adlercreutzia, Rikenella, and Clostridium) and the above metabolites.
CONCLUSION: LPD-KA modulated the intestinal flora and urinary metabolism to attenuate DN progression, with Lactobacillus, Akkermansia, Bacteroides, L-histidinol, gluconolactone, 2-ketobutyric acid, and dihydrocortisol as potential biomarkers, providing new insights into the clinical application of LPD-KA for DN treatment.
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목차
- ABSTRACT
- INTRODUCTION
- MATERIALS AND METHODS
- RESULTS
- DISCUSSION
- REFERENCES
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