인문학
사회과학
자연과학
공학
의약학
농수해양학
예술체육학
복합학
DBpia AI
AI 에이전트
AI 아이디어
AI 리더
AI 뷰어
Citeasy
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지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2025.12
- 수록면
- 528 - 535 (8page)
이용수
초록· 키워드
Conventional flow cytometers rely on sheath-flow hydrodynamic focusing and fluorescence-based detection, but their complexity, cost, and maintenance requirements limit widespread adoption. To address these limitations, we developed a disposable lab-on-a-chip flow cytometer that integrates MEMS-based microfluidics, optical trapping, and on-chip fluorescence detection within a compact format. Microchannels were fabricated using deep silicon etching and PDMS plasma bonding, enabling inertial focusing and stable laminar flow without sheath fluids. The syringe-integrated disposable chip eliminates external tubing and cleaning procedures, enabling contamination-free and user-friendly operation. For multiparametric detection, a diode laser–based optical trapping module (650-1650 nm) was coupled with a fluorescence detection unit comprising excitation optics, dichroic mirrors, and photodetectors. Using 30 μm fluorescently labeled polystyrene beads (FITC, PE), the system achieved stable trapping and fluorescence- triggered sorting with measurement errors below 5%, while providing clear and distinct detection of fluorescently labeled beads against background signals. This work demonstrates a disposable lab-on-a-chip cytometer that uniquely combines MEMS microfabrication, optical trapping, and fluorescence detection, offering a low-cost and high-precision alternative to conventional flow cytometers for clinical diagnostics, point-of-care testing(POCT), and next-generation cytometric applications.
상세정보 수정요청해당 페이지 내 제목·저자·목차·페이지정보가 잘못된 경우 알려주세요!
목차
- Abstract
- Ⅰ. INTRODUCTION
- Ⅱ. System Design, Fabrication, and Experimental Methods
- Ⅲ. Development of Optical Trapping and Sorting Module
- Ⅳ. Fluorescence Detection and Optical Sorting
- Ⅴ. Conclusion
- References
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