인문학
사회과학
자연과학
공학
의약학
농수해양학
예술체육학
복합학
DBpia AI
AI 에이전트
AI 아이디어
AI 리더
AI 뷰어
Citeasy
크롬 서지관리 다운로드
지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
피인용 0
초록·키워드
Background: The genus Bupleurum (Apiaceae) includes the medicinal species B. komarovianum,B. longiradiatum, and B. latissimum, which are often misidentified or mixed in herbal markets. Therefore, reliable molecular markers are needed for accurate species discrimination and prevention of adulterations and misuses.
Methods and Results: Complete plastid genomes (plastomes) and 45S nuclear ribosomal DNA (nrDNA) sequences of B. longiradiatum and B. latissimum were newly assembled from Illumina MiSeq data using the de novo assembly of low-coverage whole-genome sequencing method and analyzed together with previously reported B. komarovianum sequences for comparative analysis. Further comparative analysis with six additional genomes from GenBank revealed species-specific single nucleotide polymorphisms. Based on these variants, we developed six single-nucleotide polymorphism (SNP) markers and validated them using high-resolution melting (HRM) analysis. The HRM profiles clearly distinguished the three species. Phylogenetic analysis using the maximum likelihood method supported species-level clustering, with B. latissimum forming a genetically distinct clade.
Conclusions: Complete plastomes and nrDNA sequences of three Bupleurum species were assembled, and SNP markers were developed and validated for species discrimination. These markers enable accurate authentication and help prevent adulteration in herbal market.
Methods and Results: Complete plastid genomes (plastomes) and 45S nuclear ribosomal DNA (nrDNA) sequences of B. longiradiatum and B. latissimum were newly assembled from Illumina MiSeq data using the de novo assembly of low-coverage whole-genome sequencing method and analyzed together with previously reported B. komarovianum sequences for comparative analysis. Further comparative analysis with six additional genomes from GenBank revealed species-specific single nucleotide polymorphisms. Based on these variants, we developed six single-nucleotide polymorphism (SNP) markers and validated them using high-resolution melting (HRM) analysis. The HRM profiles clearly distinguished the three species. Phylogenetic analysis using the maximum likelihood method supported species-level clustering, with B. latissimum forming a genetically distinct clade.
Conclusions: Complete plastomes and nrDNA sequences of three Bupleurum species were assembled, and SNP markers were developed and validated for species discrimination. These markers enable accurate authentication and help prevent adulteration in herbal market.
본문·목차
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