인문학
사회과학
자연과학
공학
의약학
농수해양학
예술체육학
복합학
DBpia AI
AI 에이전트
AI 아이디어
AI 리더
AI 뷰어
Citeasy
크롬 서지관리 다운로드
지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록·키워드
Bacterial wilt is a devastating disease of tomato (<i>Solanum lycopersicum</i>) caused by <i>Ralstonia solanacearum</i> that severely threatens tomato production. Group III WRKY transcription factors (TFs) are implicated in the plant response to pathogen infection; however, their roles in the response of tomato to <i>R. solanacearum</i> infection (RSI) remain largely unexplored. Here, we report the crucial role of SlWRKY30, a group III SlWRKY TF, in the regulation of tomato response to RSI. <i>SlWRKY30</i> was strongly induced by RSI. <i>SlWRKY30</i> overexpression reduced tomato susceptibility to RSI, and also increased H<sub>2</sub>O<sub>2</sub> accumulation and cell necrosis, suggesting that SlWRKY30 positively regulates tomato resistance to RSI. RNA sequencing and reverse transcription-quantitative PCR revealed that <i>SlWRKY30</i> overexpression significantly upregulated pathogenesis-related protein (<i>SlPR-STH2</i>) genes <i>SlPR-STH2a</i>, <i>SlPR-STH2b</i>, <i>SlPR-STH2c</i>, and <i>SlPR-STH2d</i> (hereafter <i>SlPR-STH2a</i>/<i>b</i>/<i>c</i>/<i>d</i>) in tomato, and these <i>SlPR-STH2</i> genes were directly targeted by SlWRKY30. Moreover, four group III WRKY proteins (SlWRKY52, SlWRKY59, SlWRKY80, and SlWRKY81) interacted with SlWRKY30, and <i>SlWRKY81</i> silencing increased tomato susceptibility to RSI. Both SlWRKY30 and SlWRKY81 activated <i>SlPR-STH2a</i>/<i>b</i>/<i>c</i>/<i>d</i> expression by directly binding to their promoters. Taking these results together, SlWRKY30 and SlWRKY81 synergistically regulate resistance to RSI by activating <i>SlPR-STH2a</i>/<i>b</i>/<i>c</i>/<i>d</i> expression in tomato. Our results also highlight the potential of <i>SlWRKY30</i> to improve tomato resistance to RSI via genetic manipulations.
인공지능 문자 인식 모델을 통해 추출된 텍스트로, 일부 오타나 오류가 포함될 수 있으나 지속적으로 개선 중입니다.
오류를 발견하셨다면 해당 부분을 드래그한 후 ' 를 통해 신고해주세요.
오류를 발견하셨다면 해당 부분을 드래그한 후 ' 를 통해 신고해주세요.
최근 본 자료 전체보기
