인문학
사회과학
자연과학
공학
의약학
농수해양학
예술체육학
복합학
DBpia AI
AI 에이전트
AI 아이디어
AI 리더
AI 뷰어
Citeasy
크롬 서지관리 다운로드
지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록·키워드
Bacteria-based microbial immunotherapy shows various unique properties for tumor therapy owing to their active tropism to tumor and multiple anti-tumor mechanisms. However, its clinical benefit is far from satisfactory, which is limited by rapid systemic clearance and neutrophils-mediated immune restriction to compromise the efficacy, as well as non-specific distribution to cause toxicity. To address all these limitations, herein we reported a polyserotonin (PST) coated <i>Salmonella</i> (<i>Sal</i>) with surface adsorption of DNAzyme (Dz)-functionalized MnO<sub>2</sub> nanoparticles (DzMN) for tumor therapy. PST could facilely coat on <i>Sal</i> surface via oxidation and self-polymerization of its serotonin monomer, which enabled surface stealth to avoid rapid systemic clearance while maintaining the tumor homing effect. Upon targeting to tumor, the PST was degraded and exfoliated in response to acidic tumor microenvironment, thus liberating <i>Sal</i> to recover its anti-tumor activities. Meanwhile, the DzMN was also delivered into tumor via hitchhiking <i>Sal</i>, which could release Dz and Mn<sup>2+</sup> after tumor cells internalization. The Dz was then activated by its cofactor of Mn<sup>2+</sup> to cleave target PD-L1 mRNA, thus serving as a self-activated system for gene silencing. Combining <i>Sal</i> and Dz for immune activation and PD-L1 knockdown, respectively, anti-tumor immunotherapy was achieved with enhanced efficacy. Notably, PST coating could significantly decrease infection potential and non-specific colonization of <i>Sal</i> at normal organs, achieving high in vivo biosafety. This work addresses the key limitations of <i>Sal</i> for in vivo application via biomaterials modification, and provides a promising platform for better microbial immunotherapy.
인공지능 문자 인식 모델을 통해 추출된 텍스트로, 일부 오타나 오류가 포함될 수 있으나 지속적으로 개선 중입니다.
오류를 발견하셨다면 해당 부분을 드래그한 후 ' 를 통해 신고해주세요.
오류를 발견하셨다면 해당 부분을 드래그한 후 ' 를 통해 신고해주세요.
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