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Oxford University Press (OUP) Horticulture Research 11(1)
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    초록·키워드

    Clubroot disease caused by <i>Plasmodiophora brassicae</i> (<i>P. brassicae</i>) severely threatens the cultivation of Cruciferous plants, especially Chinese cabbage. Recently, resistance genes in plants have been reported to encode for a Ca<sup>2+</sup>-permeable channel in the plasma membrane, which can mediate the cytosolic Ca<sup>2+</sup> increase in plant cells upon pathogen attack. However, the downstream Ca<sup>2+</sup> sensor and decoder are still unknown. In this study, we identified the virulent and avirulent <i>P. brassicae</i> isolates (Pbs) of two near isogenic lines, CR 3-2 and CS 3-2, with CR 3-2 harboring clubroot resistant gene <i>BraCRa</i>. The transcriptomic analysis was then conducted with CR 3-2 after inoculating with virulent isolate PbE and avirulent isolate Pb4. From the differentially expressed genes of transcriptomic data, we identified a Ca<sup>2+</sup>-sensor encoding gene, <i>BraCBL1</i>.<i>2</i>, that was highly induced in CR 3-2 during infection by Pb4 but not by PbE. Moreover, GUS histochemical staining and subcellular localization analysis revealed that <i>BraCBL1</i>.<i>2</i> was specifically expressed in the root hair cells of <i>Arabidopsis</i> and encoded a putative Ca<sup>2+</sup> sensor localized in the plasma membrane. We also developed an assay to investigate the BraCRa-mediated hypersensitive response (HR) in tobacco leaves. The results suggest that BraCBL1.2 is involved in the BraCRa-mediated plant ETI immune response against <i>P. brassicae</i>. In addition, we verified that overexpression of <i>BraCBL1.2</i> enhanced clubroot resistance in <i>Arabidopsis</i>. Collectively, our data identified the involvement of a Ca<sup>2+</sup> sensor in BraCRa-mediated clubroot resistance in Chinese cabbage, providing a theoretical basis for further research on the resistance of Chinese cabbage to <i>P. brassicae</i>.

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