인문학
사회과학
자연과학
공학
의약학
농수해양학
예술체육학
복합학
DBpia AI
AI 에이전트
AI 아이디어
AI 리더
AI 뷰어
Citeasy
크롬 서지관리 다운로드
지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록·키워드
Abstract Background Low‐intensity pulsed ultrasound (LIPUS) has been highlighted as a potential therapy for tissue repair and regeneration. However, little is known about LIPUS effects on neuromodulation. This research was conducted to study LIPUS effect on the proliferation of human neural stem cells. Materials and methods The human SH‐SY5Y neuroblastoma cell line was used as a neural stem cell model. The well‐documented SH‐SY5Y neurogenic protocol, which involves treatment with all trans‐retinoic acid (ATRA) for 5 days and then brain‐derived neurotrophic factor (BDNF) for 7 days, was used to synchronise the growth cell cycle to G1 phase of the cell cycle before proliferation testing. Subsequently, the neural stem cells were then treated with single or triple 20‐min LIPUS exposures (Intensity I SATA : 60 mW/cm 2 , frequency: 1.5 MHz, pulse repetition: 100 Hz, and duty cycle: 20%). Cell proliferation was analysed using cell counting of β‐tubulin and neurofilament medium‐positive neural stem cells, Ki67‐cell proliferation marker and metabolic‐based assays (cell counting kit‐8 and alamarBlue). The involvement of ERK signalling was investigated by quantification of phospho‐ERK1/2 levels and cell proliferation with and without MEK/ERK inhibitor (U0126). Results The results show that LIPUS exposure(s) induced cell proliferation, as evidenced by an increase in the numbers of neural stem cells. ERK signalling is involved in LIPUS‐induced neural stem cell proliferation, as evidenced by concurrent inhibition of LIPUS‐induced phospho‐ERK levels and cell proliferation in the presence of the MEK/ERK inhibitor. Conclusion This study provides original evidence that LIPUS can stimulate neural stem/progenitor cell proliferation. LIPUS may be suggested as a sole or an adjunct therapeutic application to promote the neural stem cell pool in stem cell therapies and tissue engineering approaches for nerve repair and regeneration for the management of traumatic nerve injuries and regenerative endodontic treatment.
인공지능 문자 인식 모델을 통해 추출된 텍스트로, 일부 오타나 오류가 포함될 수 있으나 지속적으로 개선 중입니다.
오류를 발견하셨다면 해당 부분을 드래그한 후 ' 를 통해 신고해주세요.
오류를 발견하셨다면 해당 부분을 드래그한 후 ' 를 통해 신고해주세요.
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