인문학
사회과학
자연과학
공학
의약학
농수해양학
예술체육학
복합학
DBpia AI
AI 에이전트
AI 아이디어
AI 리더
AI 뷰어
Citeasy
크롬 서지관리 다운로드
지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록·키워드
Introducing high school students to the research life cycle remains a challenge within education. Glass Half Full or Empty: Illuminating the Human Transcriptome is an eight-session course designed to introduce underserved high school students in New York City to wet-lab biochemistry and molecular biology techniques. The content introduces students to the underlying principles of the molecular DNA/RNA sequencing revolution and thereafter teaches students to verify novel RNA transcript variants predicted with long-read RNA-sequencing data.1 As such, students are contributing - one transcript at a time - to a more complete version of the human transcriptome. They become researchers working within a reproducible scientific tradition, recapitulating the results of their classmates across multiple independent replicates. The eight sessions consist of two lectures, two dry labs, three wet labs, and a final poster presentation session. The lectures gradually introduce the necessary theoretical content with an emphasis on visual learning, reducing barriers caused by a lack of introductory biology coursework. The first dry lab teaches students primer design, encouraging students to become familiar with the Benchling molecular biology suite. The three wet labs build student confidence in their technique, starting with a guided-polymerase chain reaction setup, followed by a semi-guided gel electrophoresis experiment, and finally progressing to an unguided gel extraction within an open-lab session. Throughout these sessions, we maintain an undergraduate mentor to high school student ratio of 2:1, recruiting from our local Columbia undergraduate community. Through this reciprocal relationship, these mentors acquire the skills to direct and instruct future iterations of the course, allowing the program to sustainably serve its target communities. As the culmination of their experience, students spend the final session producing and presenting a poster to a diverse audience of undergraduates, graduate students, and faculty. Throughout the course's duration, students have reported high satisfaction with the course quality, and its purpose as a hands-on and unique opportunity to explore the research life cycle. Often, students report a newfound and/or renewed interest in biological research, making our work all the more rewarding. 1 Nelson, T.M.; Ghosh, S.; Postler, T.S. L-RAPiT: A Cloud-Based Computing Pipeline for the Analysis of Long-Read RNA Sequencing Data. Int. J. Mol. Sci. 2022, 23, 15851. https://doi.org/10.3390/ijms232415851 We thank the Columbia University Department of Biomedical Engineering, the Columbia University Research Cluster on Science and Subjectivity, the Columbia University Double Discovery Center, the Stephens FamilyFoundation, and the American Society for Biochemistry and Molecular Biology for their financial support of this program.
인공지능 문자 인식 모델을 통해 추출된 텍스트로, 일부 오타나 오류가 포함될 수 있으나 지속적으로 개선 중입니다.
오류를 발견하셨다면 해당 부분을 드래그한 후 ' 를 통해 신고해주세요.
오류를 발견하셨다면 해당 부분을 드래그한 후 ' 를 통해 신고해주세요.
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