인문학
사회과학
자연과학
공학
의약학
농수해양학
예술체육학
복합학
DBpia AI
AI 에이전트
AI 아이디어
AI 리더
AI 뷰어
Citeasy
크롬 서지관리 다운로드
지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록·키워드
Antimicrobial Resistance (AMR) is the next impending health crisis knocking at our doors and the biggest challenge in fighting AMR infections is the scarcity of novel and effective antibiotics. The objective of this study is to evaluate novel class of compounds called benzothiophenes for their potential antimicrobial activity. Previous studies have indicated great promise of benzothiophene derivatives as an important biological core structure. Benzo[b]thiophene core structure is present in several FDA-approved drugs, such as raloxifene, zileuton, and sertaconazole. In this study, the novel benzothiophene derivatives were synthesized using common organic synthesis methods such as Sonogashira coupling and electrophilic cyclization reactions. Proton nuclear magnetic resonance (1H-NMR), carbon nuclear magnetic resonance (13C-NMR), gas chromatography-mass spectroscopy (GC-MS), and high-performance liquid chromatography (HPLC) were used to determine compound identity and purity. The compounds were tested against gram positive bacteria (S. aureus, M. luteus, and E. faecalis), gram-negative bacteria (E. coli, and P. aeruginosa) and fungi such as C. albicans and C. tropicalis, via a broth microdilution susceptibility assay in a 96-well plate. Briefly, varying concentrations of the compound were incubated with the cells. After incubation, the plates were read using a plate reader to determine the absorbance and minimum inhibitory concentration (MIC) values. The low MIC values for the benzo[b]thiophene derivative compounds signify a promising antimicrobial activity. Further biological studies were performed to determine the toxicity of the compounds to human cell lines. Preliminary results showed that addition of some groups increased the antibacterial activity of the benzo[b]thiophene derivatives while addition of other groups decreased the activity of the compounds. In conclusion, this project will help to identify more potent benzo[b]thiophene derivatives with broader efficacy against both bacteria and fungi. This research accomplishes crucial steps toward developing potent benzo[b]thiophenes as a possible clinical candidate as an antimicrobial drug. This research has been supported by department of biology, office of undergraduate research (OUR), Hal Marcus College of Science and Engineering at UWF and Eppley foundation for research grant.
인공지능 문자 인식 모델을 통해 추출된 텍스트로, 일부 오타나 오류가 포함될 수 있으나 지속적으로 개선 중입니다.
오류를 발견하셨다면 해당 부분을 드래그한 후 ' 를 통해 신고해주세요.
오류를 발견하셨다면 해당 부분을 드래그한 후 ' 를 통해 신고해주세요.
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