인문학
사회과학
자연과학
공학
의약학
농수해양학
예술체육학
복합학
DBpia AI
AI 에이전트
AI 아이디어
AI 리더
AI 뷰어
Citeasy
크롬 서지관리 다운로드
지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록·키워드
Chlamydia trachomatis is the most prevalent sexually transmitted bacterial infection worldwide, representing a major public health concern. This obligate intracellular bacterium has a phylum-defining biphasic developmental cycle whose regulatory signals and mechanisms are still poorly understood. One protein system that has been shown to govern the cycle's regulation is the Rsb system. This system works based on the phosphorylation state of an intermediate, RsbV1, which determines the activity of a periplasmic sensor phosphatase and a terminal protein partner kinase, RsbU and RsbW respectively. Although genetic disruption of RsbU has been shown to cause a dramatic decrease in progeny production, disruption of RsbW does not, making the Rsb system's role in C. trachomatis' development unclear. We examined the importance of RsbV1's phosphorylation state by studying the effect of its overexpression on progeny production and growth with a tetracycline inducible system. We also examined the overexpression of a phosphodeficient mutant for RsbV1, RsbV1S56A, and used immunofluorescent microscopy to determine effects on morphology and size. Induction between 16 and 24 hours post infection led to a significant decrease in progeny production with overexpression of both wildtype and phosphodeficient RsbV1. Furthermore, we found that expression of RsbV1S56A led to a significant reduction in inclusion size. Future studies with different induction periods will be performed to clarify the role of RsbV1's phosphorylation state in the system, which could provide a better insight on C. trachomatis' development and potential ways to address its public health impact. This project was supported by an Institutional Development Award (IDeA) from the National Institute of General Medical Sciences of the National Institutes of Health under grant number P20 GM103418.
인공지능 문자 인식 모델을 통해 추출된 텍스트로, 일부 오타나 오류가 포함될 수 있으나 지속적으로 개선 중입니다.
오류를 발견하셨다면 해당 부분을 드래그한 후 ' 를 통해 신고해주세요.
오류를 발견하셨다면 해당 부분을 드래그한 후 ' 를 통해 신고해주세요.
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