인문학
사회과학
자연과학
공학
의약학
농수해양학
예술체육학
복합학
DBpia AI
AI 에이전트
AI 아이디어
AI 리더
AI 뷰어
Citeasy
크롬 서지관리 다운로드
지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록·키워드
Bacterial microorganisms are crucial for human health and digestion. The Bacteroides are prevalent members of the gut microbial community that are essential for polysaccharide degradation and formation of a healthy gut microbiome. In the gram-negative model organism Bacteroides thetaiotaomicron (B. theta), specialized membrane proteins known as TonB-Dependent Transporters (TBDTs) facilitate the movement of complex polysaccharides into the cell. This transport is energized by TonB proteins in the inner membrane. Previous research on the 11 identified TonB proteins in B. theta involved characterizing their role in polysaccharide utilization through gene deletions. This research showed that although the TonB7 protein (BT3192) was not present in the membrane fraction of glucose or maltose grown cells, deletion of this gene produced a lag in bacterial growth that was not rescued by complementation. This suggests that removing TonB7 from the genome disrupts some essential process(es) other than TonB interaction with the polysaccharide transporter. To achieve a ΔTonB7 strain without this phenotype, the deletion scheme was redesigned to target two highly conserved portions of the gene that are predicted to interact with the TBDT: the entire C-terminal domain (CTD) and a beta-sheet within that domain. These regional deletion strains were grown on glucose to assess growth changes compared to wild-type and ΔTonB7 B. theta. The lag phenotype was absent in both ΔCTD and Δβ-sheet TonB7 strains suggesting we have been able to construct a deletion that eliminates interactions with the TBDT but does not disrupt other processes in the bacteria. Additional growth assays on starch will inform TonB7's role in polysaccharide transport. Multi-deletion strains will also be constructed using the ΔTonB7 regional deletions to explore TBDT specificity for the 11 TonB proteins. Comparison of the B. theta TonB proteins to those encoded by other Bacteroides species suggests that TonB7 is found only in a limited number of species closely related to B. theta while TonB4 (BT2059) is highly conserved across the Bacteroides. Our novel deletion construct allowing for disruption of TonB7 function without inducing a lag on glucose enables further investigation of TonB redundancy and TBDT specificity.
인공지능 문자 인식 모델을 통해 추출된 텍스트로, 일부 오타나 오류가 포함될 수 있으나 지속적으로 개선 중입니다.
오류를 발견하셨다면 해당 부분을 드래그한 후 ' 를 통해 신고해주세요.
오류를 발견하셨다면 해당 부분을 드래그한 후 ' 를 통해 신고해주세요.
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