인문학
사회과학
자연과학
공학
의약학
농수해양학
예술체육학
복합학
DBpia AI
AI 에이전트
AI 아이디어
AI 리더
AI 뷰어
Citeasy
크롬 서지관리 다운로드
지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록·키워드
Plastic pollution is a growing global health problem that has broad research implications. This project explored the effects of microplastics (MPs, 5mm – 1μm) and nanoplastics (NPs, 999nm – 1nm) on the overall molecular structure of bovine serum albumin (BSA), a transporter of fatty acids and common in vitro protein stabilizer. As plastic breaks down in nature due to a multitude of mechanical and chemical forces, it can become small enough to pass through the blood-brain barrier and interact with blood-borne proteins. Within this study, BSA serves as a model for determining the potential extent of this interaction, which was measured using circular dichroism (CD) spectroscopy, microscopy, and preliminary protein docking computational calculations. CD spectra revealed that BSA underwent significant global structural changes after exposure to polypropylene (PP) MPs and NPs at 20˚C. CD data obtained during thermal denaturation assays revealed that while the melting temperature (Tm) of BSA decreases from 61˚C to 60˚C after exposure to plastic particles, the thermodynamics of unfolding were notably different which again indicated notable structural changes. Secondary structural analysis of BSA from CD spectra revealed that before exposure to MPs and NPs, BSA was shown to have 55.5% alpha helices, 17.4% beta sheets, 11.9% beta turns, and 15.2% random coiling. After exposure to MPs and NPs, those values changed to 57.9%, 13.6%, 15%, and 13.5% respectively. Comparing those values to docking data revealed that the potential binding pockets for PP altered the secondary and tertiary structures as well. The proposed binding affinities for PP in BSA revealed that it binds strongly enough to the protein for its natural efficiency to be impeded due to decreased availability to transport. This work was financially supported by St. Mary's College of Maryland, NSF MRI grant #1919581, and the Sherman Fairchild Foundation SEP grant.
인공지능 문자 인식 모델을 통해 추출된 텍스트로, 일부 오타나 오류가 포함될 수 있으나 지속적으로 개선 중입니다.
오류를 발견하셨다면 해당 부분을 드래그한 후 ' 를 통해 신고해주세요.
오류를 발견하셨다면 해당 부분을 드래그한 후 ' 를 통해 신고해주세요.
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