인문학
사회과학
자연과학
공학
의약학
농수해양학
예술체육학
복합학
DBpia AI
AI 에이전트
AI 아이디어
AI 리더
AI 뷰어
Citeasy
크롬 서지관리 다운로드
지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록·키워드
The bioactive components of human milk play a crucial role in its positive impact on newborn development. Extracellular vesicles (EVs) are small, membrane-enclosed, non-replicating vehicles secreted by lactocytes that may be important in newborn nutrition. The functions and dietary bioavailability of EVs may depend on their lipid composition. Oxylipins are polyunsaturated fatty acids (PUFA) derived metabolites present in EVs that may influence EV bioavailability by modulating the permeability of biological barriers, like the intestine. Objective. This research aims to characterize the PUFA derived metabolite content of human milk EVs. Methods. Mature human milk (n = 20) was obtained from Prolacta Bioscience (City of Industry, CA). EVs were isolated from human milk using differential centrifugation and concentrated using a precipitation reagent. Lipids were extracted from EVs and human milk using methanol and hexane, and then subjected to solid phase extraction to recover free oxylipins. Liquid chromatography-mass spectrometry was used to identify oxylipins and the oxylipins were quantified with internal standards. The relative amounts of oxylipins were computed and descriptive statistics were determined using Wilcoxon test for non normal distribution. Results. The EV isolation was characterized in accordance with guidance from the Minimal Information for the Study of EVs. Relative to total oxylipin content, the relative amounts of oxylipins derived from arachidonic acid (AA), linoleic acid (LA), docosahexaenoic acid (DHA), and eicosapentaenoic acid (EPA) in EVs were 8.7%, 88%, 2.7%, and 0.18%, respectively. In whole human milk, the relative amounts of oxylipins derived from AA, LA, DHA, and EPA were 12%, 82%, 5.4%, and 0.89%, respectively. AA derived oxylipin content was decreased ∼25% (p = 0.002) in EVs compared to whole human milk. DHA derived oxylipin content in EVs was ∼50% lower (p < 0.001), and EPA derived oxylipin content was decreased ∼80% relative to whole human milk (p < 0.001). The relative amount of oxylipins derived from omega-6 fatty acids was greater than the relative amount of oxylipins from omega-3 fatty acids in EVs and in human milk. In EVs, 2.9% of total oxylipins were derived from omega-3 fatty acids compared to 6.3% in whole human milk (p < 0.001). In EVs, 97% of oxylipins were derived from omega-6 fatty acids compared to 93.7% in whole human milk (p < 0.001). Conclusions. The free oxylipin content of EVs differs between EVs and whole human milk. This finding may suggest selective partitioning or non-stochastic distribution of oxylipins in milk and milk derived EVs from lactocytes. The prominent increase in total omega-6 derived oxylipins in EVs suggests their importance in the bioavailability of dietary EVs. The study was funded by the USDA (2021-38420-34941 and 2020-67018-33315).
인공지능 문자 인식 모델을 통해 추출된 텍스트로, 일부 오타나 오류가 포함될 수 있으나 지속적으로 개선 중입니다.
오류를 발견하셨다면 해당 부분을 드래그한 후 ' 를 통해 신고해주세요.
오류를 발견하셨다면 해당 부분을 드래그한 후 ' 를 통해 신고해주세요.
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