인문학
사회과학
자연과학
공학
의약학
농수해양학
예술체육학
복합학
DBpia AI
AI 에이전트
AI 아이디어
AI 리더
AI 뷰어
Citeasy
크롬 서지관리 다운로드
지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록·키워드
Flaviviruses are a family of RNA viruses that are commonly found in arthropods and can be transmitted to both animal and human hosts. A large portion of these flaviviruses are human pathogens, infecting up to 400 million people each year. These include Yellow Fever, Dengue, West Nile, Zika, and Powassan viruses. Transfer between hosts, combined with the high error rate of the RNA-dependent RNA polymerases found in these virus, can drive increased rates of mutation and potentially increase viral pathogenicity. Current evidence indicates viral RNA genomes are modified post-transcriptionally, and that these modifications could affect viral RNA stability and replication. This work explores the effect these epigenetic RNA modifications have on the speed and accuracy of RNA transcription by flavivirus RNA-dependent RNA polymerase (RdRp). To examine the effect RNA modifications have on transcription rate and fidelity, we have designed and developed an in vitro assay, using enzyme purified from expression constructs of the flavivirus NS5 RdRp and model RNA transcription templates containing modifications that have been determined to be present in these viral genomes. Using this system, we are measuring the effects these functional group modifications have on how the flavivirus RNA polymerase reads the modified RNA template. Understanding the impact RNA modifications have on flavivirus RNA synthesis will further illuminate its effect on viral replication and evolution. This work was supported by a Scialog grant to K. Koutmou from the Research Corporation and the Albion College Foundation for Undergraduate Research.
인공지능 문자 인식 모델을 통해 추출된 텍스트로, 일부 오타나 오류가 포함될 수 있으나 지속적으로 개선 중입니다.
오류를 발견하셨다면 해당 부분을 드래그한 후 ' 를 통해 신고해주세요.
오류를 발견하셨다면 해당 부분을 드래그한 후 ' 를 통해 신고해주세요.
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