인문학
사회과학
자연과학
공학
의약학
농수해양학
예술체육학
복합학
지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록·키워드
Photosynthetic organisms have evolved an essential energy-dependent quenching (qE) mechanism to avoid any lethal damages caused by high light. While the triggering mechanism of qE has been well addressed, candidates for quenchers are often debated. This lack of understanding is because of the tremendous difficulty in measuring intact cells using transient absorption techniques. Here, we have conducted femtosecond pump-probe measurements to characterize this photophysical reaction using micro-sized cell fractions of the green alga Chlamydomonas reinhardtii that retain physiological qE function. Combined with kinetic modeling, we have demonstrated the presence of an ultrafast excitation energy transfer (EET) pathway from Chlorophyll a (Chl a) Q<sub>y</sub> to a carotenoid (car) S<sub>1</sub> state, therefore proposing that this carotenoid, likely lutein1, is the quencher. This work has provided an easy-to-prepare qE active thylakoid membrane system for advanced spectroscopic studies and demonstrated that the energy dissipation pathway of qE is evolutionarily conserved from green algae to land plants.
#Photoprotection
#Chlamydomonas reinhardtii
#Non-photochemical quenching
#Quenching (fluorescence)
#Ultrafast laser spectroscopy
#Biophysics
#Chlamydomonas
#Photosynthesis
#Thylakoid
#Photochemistry
#Femtosecond
#Chemistry
#Chlorophyll fluorescence
#Fluorescence
#Electron transport chain
#Chloroplast
#Biology
#Physics
#Biochemistry
#Spectroscopy
#Optics
#Mutant
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