인문학
사회과학
자연과학
공학
의약학
농수해양학
예술체육학
복합학
DBpia AI
AI 에이전트
AI 아이디어
AI 리더
AI 뷰어
Citeasy
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록·키워드
This paper presents an <i>in vitro</i> evaluation of antitumor properties through producing short-chain fatty acids and inducing interleukin 12. In addition, it offers the most important and functional probiotic properties of 24 <i>Lactobacillus gasseri</i>, <i>Lactiplantibacillus plantarum, Lactobacillus acidophilus,</i> and <i>Limosilactobacillus fermentum</i> strains isolated from humans, foods, and fermented foods. To this end, survival in an acidic environment (pH = 2.5), tolerance in bile salt, viability in the presence of pepsin-pancreatin, adhesion percentage, antibiotic resistance, auto-aggregation, and potential percentage of co-aggregation are studied in contact with three human intestinal pathogens. These pathogens are <i>Escherichia coli</i> O157: H7 NCTC 12900, <i>Salmonella enterica</i> subsp<i>. enterica</i> ATCC 13076, and <i>Listeria monocytogenes</i> ATTC 7644. Also, <i>in vitro</i> induction amount of IL-12 in mouse splenocytes is investigated to evaluate antitumor properties by 19 strains of <i>L. gasseri</i> and <i>L. plantarum</i> along with the development of short-chain fatty acids (SCFA) by 5 strains of <i>L. fermentum</i> and <i>L. acidophilus</i>. Gas Chromatography Flame Ionization Detector (GC-FID) and enzyme-linked immunosorbent assay (ELISA) were used to measure short-chain fatty acids and IL-12, respectively. All strains had high viability under acidic conditions. The highest levels of pancreatin and pepsin resistance were found in strains LF56, LF57, LF55, OF, and F and strains LF56, LF57, and A7, respectively. All strains except LF56 had high resistance to bile salts. <i>L. gasseri</i> 54C had the highest average adhesion score (hydrophobicity) of 62.9 % among 19 strains<i>.</i> Despite the susceptibility of different strains of <i>L. plantarum</i> to the tested antibiotics, M8 and M11, S2G, A7, LF55, LF57, and 5G were resistant to kanamycin and chloramphenicol, respectively. Also, 21G was resistant to ampicillin, LF56 to tetracycline and M8, and M11, LF56, and 21G to Erythromycin. In addition, <i>L. gasseri</i> showed moderate resistance to ampicillin, erythromycin, and tetracycline, while <i>L. fermentum</i> ATCC 9338 showed good resistance to ampicillin, erythromycin, and chloramphenicol. In this respect, <i>L. plantarum</i> LF56 and <i>gasseri</i> 54C had the highest average auto-aggregation and co-aggregation against three pathogenic bacteria, respectively. The highest and lowest levels of acetic acid as short-chain fatty acids were produced by <i>L. fermentum</i> 19SH isolated from Horre 41.62 and <i>L. fermentum</i> 21SH from fermented seeds 27.047, respectively<i>.</i> Moreover, <i>L. fermentum,</i> with the OF code of traditional-fermented food origin, produced the most isobutyric acid, butyric acid, and valeric acid, with values of 0.6828, 0.74165, and 0.49915 mmol, respectively. <i>L. fermentum</i> isolated from the human origin with code F produced the most isovaleric acid of 1.1874 mmol. All the tested strains produced good propionic acid except <i>L. fermentum</i> 21SH from fermented seeds. Among strains, <i>L. plantarum</i> M11 isolated from milk and <i>L. gasseri</i> 52B from humans had the highest <i>in vitro</i> induction of IL-12, which is probably related to their cell wall compositions and structure.
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