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EDP Sciences BIO Web of Conferences 129
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    초록·키워드

    BackgroundTerrestrial isopods (Oniscidea) are a successful group of terrestrial crustaceans, partly due to their finely structured exoskeletal cuticle.The cuticle is an elaborate extracellular matrix deposited by epidermal cells and consists of several layers.The epicuticle, the thinnest and outermost layer, forms remarkably diverse and complex structures, such as scales and other micrometre-sized ornaments that influence the surface properties of the cuticle and the interaction of isopods with their environment [1, 2].However, not much is known about the formation of the epicuticle and these structures.The aim of this study was to develop a simple protocol to investigate dorsal epicuticle formation in terrestrial isopods during molt.For this purpose, a combination of scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM) was applied to image the deposition of the new epicuticle during molt in the terrestrial isopod Porcellio scaber.

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