인문학
사회과학
자연과학
공학
의약학
농수해양학
예술체육학
복합학
DBpia AI
AI 에이전트
AI 아이디어
AI 리더
AI 뷰어
Citeasy
크롬 서지관리 다운로드
지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록·키워드
Shigella causes millions of cases and hundreds of thousands of deaths annually, mainly affecting children in impoverished countries. Shigellosis outbreaks have been linked to waterborne transmission, and chlorination is commonly used to disinfect water. However, the emergence of chlorine-resistant Shigella spp. is a concern. Therefore, it is imperative to develop an alternative antibacterial agent such as endolysin against the Shigella spp. In this study, the Escherichia phage KW1E_UTAR gene encoding for endolysin was successfully cloned and expressed in Escherichia coli pET-28a. The purified endolysin was characterized, and its antibacterial activity was determined. The purified endolysin remained stable between pH 4 and 9, and at a broad range of temperature (4°C–75°C). The endolysin's storage stability was tested for one week, one month, and three months at different temperatures (4°C, −20°C, and −80°C). Despite over 50% drop in the lytic activity, the purified endolysin outperformed the commercial lysozyme. Besides, all four Shigella spp. tested were susceptible to the purified endolysin, with Shigella flexneri being the most prominent. Subsequently, the purified endolysin’s effectiveness in removing S. flexneri from spiked water samples was evaluated. After an hour of treatment, the number of viable bacteria in the water samples was reduced by 99.9%. Therefore, endolysin could be effective at eradicating Shigella spp. in various water sources.
인공지능 문자 인식 모델을 통해 추출된 텍스트로, 일부 오타나 오류가 포함될 수 있으나 지속적으로 개선 중입니다.
오류를 발견하셨다면 해당 부분을 드래그한 후 ' 를 통해 신고해주세요.
오류를 발견하셨다면 해당 부분을 드래그한 후 ' 를 통해 신고해주세요.
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