인문학
사회과학
자연과학
공학
의약학
농수해양학
예술체육학
복합학
DBpia AI
AI 에이전트
AI 아이디어
AI 리더
AI 뷰어
Citeasy
크롬 서지관리 다운로드
지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록·키워드
Nanoparticles (approximately 100 nm in diameter) composed of lipid layers containing drugs or biologically active substances are attracting increasing attention in various fields, including medicine, as well as for signal transduction between cells. However, the separation of such nanoparticles <i>via</i> conventional HPLC is challenging, often resulting in the clogging and collapse of nanoparticles, as well as a low separation efficiency. Thus far, no HPLC column capable of efficiently separating two types of 100 nm-sized nanoparticles in a short time has been reported. In this study, a poly-Lys-modified monolithic column was prepared for nanoparticle analysis <i>via</i> HPLC using anticancer drug-encapsulated nanoparticles (Doxil®) and small extracellular vesicles (sEVs) to examine their elution behaviors. The zeta potentials of Doxil® and the sEVs were -24.4 and -45.5 V, respectively. A column with a low surface coverage (0.96 mg mL<sup>-1</sup>) of poly-Lys adsorbed the nanoparticles but did not elute them, whereas a column with a high surface coverage (2.06 mg mL<sup>-1</sup>) of poly-Lys retained these nanoparticles owing to the ion-exchange effect; sEVs with highly negative charges were strongly retained in the column. Using gradient elution with different 2-amino-2-hydroxymethyl-1,3-propanediol concentrations in the mobile phase, the two types of nanoparticles (Doxil® and sEVs) were eluted and successfully separated within 10 min. Thus, the developed column is a valuable tool for evaluating the safety and performance of larger-sized nanoparticles.
인공지능 문자 인식 모델을 통해 추출된 텍스트로, 일부 오타나 오류가 포함될 수 있으나 지속적으로 개선 중입니다.
오류를 발견하셨다면 해당 부분을 드래그한 후 ' 를 통해 신고해주세요.
오류를 발견하셨다면 해당 부분을 드래그한 후 ' 를 통해 신고해주세요.
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