인문학
사회과학
자연과학
공학
의약학
농수해양학
예술체육학
복합학
DBpia AI
AI 에이전트
AI 아이디어
AI 리더
AI 뷰어
Citeasy
크롬 서지관리 다운로드
지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록·키워드
Abstract Background The mammalian gastrointestinal tract hosts a complex microbiome essential for sustaining host health, particularly during pivotal stages such as weaning in piglets. Weaning represents a significant stressor, leading to substantial shifts in the gut microbiota composition and functionality. This study investigated the impact of weaning stress on the gut microbiome and metabolite profiles of piglets, focusing on how methionine supplementation influences gut health and physiological development. Results A multiomics approach that integrates metagenomics, metabolomics, culturomics, and transcriptomics was employed to characterize the gut microbiota before and after weaning. During the weaning period, weaning stress was characterized by a reduction in the abundance of beneficial bacteria in the gut, particularly a significant decrease in commensal Lactobacillus species, such as L. mucosae , L. reuteri , and L. amylovorus . Metabolomic analysis further revealed reductions in methionine and other metabolites associated with methionine metabolism and reductions in branched-chain amino acids (BCAAs) and lipid-related metabolites. Analysis of culture supernatants from Lactobacillus isolates demonstrated that these commensal Lactobacillus produced methionine, methionine-related metabolites, and BCAAs, highlighting a close relationship between methionine and commensal Lactobacillus abundance under weaning stress. Moreover, methionine supplementation in intestinal epithelial cells under methionine-deficient conditions led to the upregulation of genes related to methionine and pyruvate metabolism. Conclusions Weaning stress results in the simultaneous reduction of both commensal Lactobacillus abundance and methionine levels in the piglet gut, with a significant inter-relationship between these factors. Methionine supplementation shows potential in mitigating gut dysbiosis and metabolic disruptions induced by weaning stress. Graphical abstract
인공지능 문자 인식 모델을 통해 추출된 텍스트로, 일부 오타나 오류가 포함될 수 있으나 지속적으로 개선 중입니다.
오류를 발견하셨다면 해당 부분을 드래그한 후 ' 를 통해 신고해주세요.
오류를 발견하셨다면 해당 부분을 드래그한 후 ' 를 통해 신고해주세요.
최근 본 자료 전체보기
