인문학
사회과학
자연과학
공학
의약학
농수해양학
예술체육학
복합학
지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록·키워드
This article presents the data of studies on optimization of conditions for culturing the causative agent of bovine adenovirus infection on continuous cell culture lines. The work used the reference strain of bovine adenovirus subgroup 1 “Adeno III WBR - 1”; cell lines of newborn Syrian hamster kidney (BHK-21/13), calf kidney (Taurus-1), endothelium of coronary vessels of the embryo cow (KST), epithelium of the lung embryo of cattle (LEK) and Madin-Darby bovine kidney (MDBK). It was found that with a stationary cultivation method, the adenovirus strain quickly adapted to the continuous cell culture lines BHK-21/13, Taurus-1, KST and MDBK. Among the continuous cell cultures, the MDBK (0.0001 MOI/cell) demonstrated the highest sensitivity to infection with the adenovirus strain. The maximum accumulation of the adenovirus strain in the culture fluid was observed 24 hours after infection. Comparative assessment of the level of strain accumulation in the culture fluid obtained using the MDBK cell line after various methods of its cultivation showed that the maximum titer of the virus strain ((6.55±0.21) lg TCID50/cm 2 ) was observed with the roller method of cell cultivation. The obtained results can be used to develop a test system for the purpose of identifying pathogens of adenovirus infection in cattle.
인공지능 문자 인식 모델을 통해 추출된 텍스트로, 일부 오타나 오류가 포함될 수 있으나 지속적으로 개선 중입니다.
오류를 발견하셨다면 해당 부분을 드래그한 후 ' 를 통해 신고해주세요.
오류를 발견하셨다면 해당 부분을 드래그한 후 ' 를 통해 신고해주세요.