인문학
사회과학
자연과학
공학
의약학
농수해양학
예술체육학
복합학
DBpia AI
AI 에이전트
AI 아이디어
AI 리더
AI 뷰어
Citeasy
크롬 서지관리 다운로드
지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록·키워드
Abstract Xanthomonas is a genus of plant-associated Gram-negative bacteria which infect more than 400 plant species. A characteristic feature of Xanthomonas bacteria is the production of yellow membrane-bound pigments called xanthomonadins. Xanthomonadins are phospholipid-like bio-macromolecules located at the outer membrane. The chemical structure and biosynthetic mechanism of xanthomonadin production remain to be fully elucidated. In this study, a total of 24 Xanthomonas strains from five different species were collected for methylated ester of aryl polyene (MEAP) preparation. High-Performance Liquid Chromatography (HPLC) and Quadrupole Time-of-Flight Mass Spectrometry (Q-TOF–MS) analysis identified three dominant MEAPs, methylated di-brominated MEAP-1, di-brominated MEAP-2, and mono-brominated MEAP-3. MEAP-1 corresponded to the previously reported aryl polyene in Xanthomonas juglandis XJ103. The 24 Xanthomonas strains could be grouped into three categories based on their MEAP profiles. Further, bacterial ooze was collected from X . oryzae pv. oryzae ( Xoo )-infected rice leaves and MEAP was prepared. The dominant MEAP in the Xoo ooze was MEAP-2. This is the first demonstration of in-planta MEAP production during plant infection of any Xanthomonas pathogen. In addition, a xan biosynthetic cluster, which is responsible for xanthomonadin biosynthesis, and the roles of the individual xan genes in MEAP biosynthesis were studied via deletion and subsequent complementation analysis. HPLC and Q-TOF–MS analysis identified the essential genes for MEAP biosynthesis, as well as the genes associated with methylation and bromination. These results provide new insights into the structural diversity of Xanthomona s MEAPs and xanthomonadin biosynthetic mechanisms.
인공지능 문자 인식 모델을 통해 추출된 텍스트로, 일부 오타나 오류가 포함될 수 있으나 지속적으로 개선 중입니다.
오류를 발견하셨다면 해당 부분을 드래그한 후 ' 를 통해 신고해주세요.
오류를 발견하셨다면 해당 부분을 드래그한 후 ' 를 통해 신고해주세요.
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