인문학
사회과학
자연과학
공학
의약학
농수해양학
예술체육학
복합학
지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록·키워드
Abstract Virus-based gene expression is a simple and powerful approach for functional genetic studies in plants. Here, sugarcane mosaic virus (SCMV, Potyvirus sacchari ) was engineered as a dual gene expression vector for simultaneous expression of two heterologous proteins in maize plants. Inoculation of the full-length cDNA clone of SCMV from agro-infiltrated Nicotiana benthamiana resulted in a rapid systemic infection in maize. To assess the possibility of SCMV as the gene expression vector, the marker gene GFP or GUS was inserted into either the NIb/CP or P1/HC-Pro junction site of SCMV to produce single-gene expression vectors. The results showed that these engineered SCMV vectors permitted efficient gene expression in systemically infected leaves and had the genetic capacity of inserts of more than 1800 bp, suggesting that both junction sites are suitable for heterologous gene insertion and expression. Furthermore, two different genes GFP and mCherry could be expressed simultaneously by engineering them into either NIb/CP or P1/HC-Pro junction sites of the same vector. These results clearly demonstrate the suitability of SCMV as a transient dual gene expression vector for maize plants.
인공지능 문자 인식 모델을 통해 추출된 텍스트로, 일부 오타나 오류가 포함될 수 있으나 지속적으로 개선 중입니다.
오류를 발견하셨다면 해당 부분을 드래그한 후 ' 를 통해 신고해주세요.
오류를 발견하셨다면 해당 부분을 드래그한 후 ' 를 통해 신고해주세요.