인문학
사회과학
자연과학
공학
의약학
농수해양학
예술체육학
복합학
DBpia AI
AI 에이전트
AI 아이디어
AI 리더
AI 뷰어
Citeasy
크롬 서지관리 다운로드
지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록·키워드
Downy mildew is a major disease that significantly impacts the yield and quality of <i>Brassica rapa</i>. While histone deacetylase (<i>HDAC)</i> family members are implicated in stress responses, their role in regulating downy mildew resistance in <i>B. rapa</i> remains unclear. Herein, we treated the susceptible <i>B. rapa</i> line R32 with Trichostatin A (TSA), a potent <i>HDAC</i> inhibitor. Notably, TSA application significantly enhanced the susceptibility of <i>B. rapa</i> seedlings to downy mildew infection, demonstrating that <i>HDAC</i> plays a crucial role in mediating resistance against this pathogen. Subsequently, we conducted phylogenetic analysis of <i>HDAC</i> family members and performed high-throughput sequencing to assess <i>HDAC</i> gene expression patterns in the resistant (R31) and susceptible (R32) lines following downy mildew inoculation. Notably, the expression of <i>BrHDA6</i> was significantly higher in the resistant line R31 compared to the susceptible line R32, suggesting its potential role in disease resistance. Using a genetic transformation system, we generated stable transgenic <i>B. rapa</i> plants overexpressing or silenced for <i>BrHDA6</i>. Inoculation with the downy mildew pathogen revealed that <i>BrHDA6</i> positively regulates disease resistance. Modification omics and parallel reaction monitoring analysis demonstrated that BrHDA6 directly reduces the acetylation level of sulphotransferase 12 (BrSOT12), which likely enhances sulfotransferase activity, consequently boosting salicylic acid production during downy mildew infection. Interaction between BrHDA6 and BrSOT12 was further validated through yeast two-hybrid and dual-luciferase assays. Our study reveals that <i>BrHDA6</i> confers downy mildew resistance in <i>B. rapa</i> through nonhistone protein deacetylation of BrSOT12, uncovering a novel regulatory mechanism in plant-pathogen interactions.
인공지능 문자 인식 모델을 통해 추출된 텍스트로, 일부 오타나 오류가 포함될 수 있으나 지속적으로 개선 중입니다.
오류를 발견하셨다면 해당 부분을 드래그한 후 ' 를 통해 신고해주세요.
오류를 발견하셨다면 해당 부분을 드래그한 후 ' 를 통해 신고해주세요.
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