인문학
사회과학
자연과학
공학
의약학
농수해양학
예술체육학
복합학
DBpia AI
AI 에이전트
AI 아이디어
AI 리더
AI 뷰어
Citeasy
크롬 서지관리 다운로드
지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록·키워드
Abstract Background The rise of antimicrobial resistance among bacteria necessitates the exploration of alternative therapeutic strategies. One potential approach is combining antibiotics with vitamins to improve antimicrobial efficacy. This study investigates the synergistic effect of ceftazidime and vitamin C against Pseudomonas aeruginosa using Drosophila melanogaster larvae as an in vivo model. Results A series of microbiological, phenotypic, and molecular assays were conducted to evaluate the antibacterial effects of ceftazidime combined with vitamin C in the Drosophila model. Ceftazidime’s minimum inhibitory concentration (MIC) decreased from 12.5 to 6.25 µM when combined with vitamin C, according to the microdilution checkerboard assay. According to in vivo tests, D. melanogaster larvae treated with the antibiotic–vitamin C combination had a significantly higher survival rate ( p < 0.05) than the infection control group. Colony-forming unit analysis on cetrimide agar medium showed no bacterial growth in the group treated with ceftazidime and vitamin C. Additionally, gene expression analysis demonstrated that ceftazidime treatment alone decreased Dpt gene expression through the IMD pathway, whereas the combination treatment elevated Dpt expression, reflecting the immunostimulant properties of vitamin C. Conclusion This study shows enhanced efficacy of ceftazidime when combined with vitamin C in the D. melanogaster larvae model. These findings parallel results from mammalian systems and support the use of Drosophila as a valuable pre-screening model for drug repurposing efforts aimed at combating antimicrobial resistance.
인공지능 문자 인식 모델을 통해 추출된 텍스트로, 일부 오타나 오류가 포함될 수 있으나 지속적으로 개선 중입니다.
오류를 발견하셨다면 해당 부분을 드래그한 후 ' 를 통해 신고해주세요.
오류를 발견하셨다면 해당 부분을 드래그한 후 ' 를 통해 신고해주세요.
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