인문학
사회과학
자연과학
공학
의약학
농수해양학
예술체육학
복합학
DBpia AI
AI 에이전트
AI 아이디어
AI 리더
AI 뷰어
Citeasy
크롬 서지관리 다운로드
지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록·키워드
Pseudomonas aeruginosa (Pa) is an opportunistic and increasingly antibiotic-resistant Gram-negative bacterium that is one of the major causes of chronic nosocomial infections.The colonization of Pa within a host is often linked to the bioavailability of nutrients, such as iron, and Pa has multiple iron acquisition pathways that allow it to adapt readily to the variety of environments it may encounter within a human host.Pa responds to these dynamic environments through the use of two-component signal transduction systems (TCSs), and previous studies have identified a biofilm-and quorum-sensing TCS known as BqsRS (also known as CarRS) that regulates biofilm formation and decay in Pa through the sensing of extracytoplasmic Fe2+ and Ca2+.In this work, we characterize the BqsRS system to understand its function and selectivity.Using biophysical approaches, we show that the membrane His kinase BqsS binds a single iron ion at a dimeric interface using a unique ligation scheme.Binding of ferrous iron elicits activity changes in the His kinase to activate the response regulator, BqsR.We have determined the structure of the BqsR receiver domain to 1.3 resolution and have modeled its DNA-binding domain.Interestingly, BqsR shows a preference for binding iron itself, which has not been observed in other OmpR-like response regulators.Importantly, using in vitro DNA-binding assays, we show that BqsR can target the Pa ferrous iron uptake operon feo.Transcriptomic changes in PAO1 show that feo is among among the most differentially regulated genes by the BqsRS sytem.Taken together, these data suggest that the BqsRS system plays an important role in iron homeostasis of this infectious pathogen.
인공지능 문자 인식 모델을 통해 추출된 텍스트로, 일부 오타나 오류가 포함될 수 있으나 지속적으로 개선 중입니다.
오류를 발견하셨다면 해당 부분을 드래그한 후 ' 를 통해 신고해주세요.
오류를 발견하셨다면 해당 부분을 드래그한 후 ' 를 통해 신고해주세요.
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