인문학
사회과학
자연과학
공학
의약학
농수해양학
예술체육학
복합학
DBpia AI
AI 에이전트
AI 아이디어
AI 리더
AI 뷰어
Citeasy
크롬 서지관리 다운로드
지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록·키워드
Translation by the ribosome is required for cell growth, development, and response to external stimuli.Translation is primarily regulated at the initiation phase, which begins when several eukaryotic initiation factors (eIFs) load onto the small ribosomal (40S) subunit to form a pre-initiation complex (PIC).The PIC is then thought to bind the 5 0 end of mRNA in an open conformation that enables mRNA loading and subsequent scanning through the 5 0 -untranslated region (5 0 -UTR) until it locates the start codon.Upon start-codon recognition, the PIC closes to arrest scanning.mRNA attachment, scanning, and start-codon recognition together comprise mRNA recruitment, the defining feature of translation initiation in eukaryotes.eIF3 is a multisubunit complex that is required for mRNA recruitment.And yet, how eIF3 or its constituent subunits contribute mechanistically to mRNA recruitment remains unknown.We leverage two eIF3 mutations that target either the entire complex (eIF3a/b Degron) or disrupt binding of two of its subunits (eIF3i/g) to the remainder of the complex (eIF3i DDKK).This latter mutation severely disrupts regions of eIF3 that bind near the mRNA-entry-channel (mEnC) of the ribosome.Using ribosome profiling, we have identified specific mRNAs whose translation is particularly sensitive to disruption of either the full eIF3 complex or its mEnC arm.However, it remains unclear which specific mRNA sequences drive this sensitivity.To ask whether the 5 0 UTRs of these sensitive mRNAs are responsible for these effects, we have constructed Luciferase reporter plasmids containing the 5 0 UTR and first 20 codons of sensitive mRNAs and have begun testing whether these regions disrupt Luciferase expression in eIF3a/b Degron and eIF3i DDKK cells.These experiments will illuminate the role of 5 0 -UTR sequences in conferring sensitivity to disruption of eIF3 or its mEnC arm, or whether other sequences within an mRNA also contribute to these effects.
인공지능 문자 인식 모델을 통해 추출된 텍스트로, 일부 오타나 오류가 포함될 수 있으나 지속적으로 개선 중입니다.
오류를 발견하셨다면 해당 부분을 드래그한 후 ' 를 통해 신고해주세요.
오류를 발견하셨다면 해당 부분을 드래그한 후 ' 를 통해 신고해주세요.
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