인문학
사회과학
자연과학
공학
의약학
농수해양학
예술체육학
복합학
DBpia AI
AI 에이전트
AI 아이디어
AI 리더
AI 뷰어
Citeasy
크롬 서지관리 다운로드
지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록·키워드
Normal cells express functional tumor suppressor WW domain-containing oxidoreductase (WWOX), designated WWOXf.UV irradiation induces WWOXf cells to undergo bubbling cell death (BCD) -an event due to overproduction of nuclear nitric oxide (NO) gas that pushes the nuclear and cell membranes to form one or two bubbles at room temperature (22 C) and below.When WWOX-deficient or -dysfunctional (WWOXd) cells are exposed to UV and/or cold shock, the cells undergo nuclear pop-out explosion death (POD) without NO.We aimed to determine whether BCD can be converted to POD.WWOXf and WWOXd cells were exposed to UV followed by measuring BCD or POD by time-lapse microscopy at 4, 22, or 37 C to visualize morphological changes.UV induces internucleosomal DNA fragmentation during apoptosis at 37oC, but no DNA fragmentation in BCD and POD at lower temperatures.UV induced co-translocation of the WWOX/ TRAF2 complex to the nuclei, in which the prosurvival TRAF2 blocked the proapoptotic WWOX via its zinc finger domain.Without WWOX, TRAF2 did not relocate to the nuclei.Cold shock caused the dissociation of the WWOX/TRAF2 complex in the nucleus needed for BCD.In contrast, the formation of the WWOX/TRAF2 complex, plus p53, was strengthened at 37 C required for apoptosis.Clathrin inhibitor pitstop 2 blocks endocytosis of serum MIF and complement proteins, and exogenous EGFP and WWOX-EGFP cDNA constructs in UV-treated cells, which results in conversion of UV-mediated BCD to POD in WWOXf cells at 22oC.In conclusion, functional WWOX is needed for UV-mediated BCD, which requires endocytosis of serum MIF and certain complement proteins to support NO generation in the nucleus.Without endocytosis, BCD turns into POD due to failure in nuclear NO production.
인공지능 문자 인식 모델을 통해 추출된 텍스트로, 일부 오타나 오류가 포함될 수 있으나 지속적으로 개선 중입니다.
오류를 발견하셨다면 해당 부분을 드래그한 후 ' 를 통해 신고해주세요.
오류를 발견하셨다면 해당 부분을 드래그한 후 ' 를 통해 신고해주세요.
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