인문학
사회과학
자연과학
공학
의약학
농수해양학
예술체육학
복합학
DBpia AI
AI 에이전트
AI 아이디어
AI 리더
AI 뷰어
Citeasy
크롬 서지관리 다운로드
지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록·키워드
Fatty acids (FAs) are a class of diverse biomolecules that are essential for a wide variety of biological processes. Recently, ω-3 and ω-6 polyunsaturated FAs (PUFAs) have received a lot of attention for their benefit and essentiality to human health. Their demand is constantly increasing alongside the ever-decreasing availability of sources of natural PUFAs, such as some plants and marine ecosystems, which are now endangered by agribusiness and climate change. Most of the novel microbial synthetic platforms to produce ω-3 and ω-6 PUFAs (e.g. yeast, microalgae, oleaginous microorganisms, bacteria etc.) are costly. On the other hand, industrial processes based on chemical total synthesis of PUFAs is often challenging. Here, we propose an innovative and bio-sustainable method to make ω-3 and ω-6 PUFAs by building a cell-factory in Crithidia fasciculata, using genetic manipulation of endogenous FA desaturases and elongases, supplementation of the culture media with cheap sources of fats (such as used cooking oils), and culturing temperature reduction. In these unusual environments, C. fasciculata show high adaptability: the cells grow rapidly producing large biomass from small volumes of culture, scavenge and remodel unusual fat sources, as well as biosynthesising high value FAs and PUFAs de novo. By genetic manipulation, allowing overexpression of either the endogenous Δ6-desaturases or Δ4-desaturases, in conjunction with the endogenous elongase Elo4, high value ω-6 and ω-3 PUFAs were yielded to a maximum of 50% of the total FA content of the cells. These bioengineered C. fasciculata provide a low-cost and bio-sustainable platform to increase the production of essential PUFAs which can be introduced into the food chain at lower costs.
인공지능 문자 인식 모델을 통해 추출된 텍스트로, 일부 오타나 오류가 포함될 수 있으나 지속적으로 개선 중입니다.
오류를 발견하셨다면 해당 부분을 드래그한 후 ' 를 통해 신고해주세요.
오류를 발견하셨다면 해당 부분을 드래그한 후 ' 를 통해 신고해주세요.
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