인문학
사회과학
자연과학
공학
의약학
농수해양학
예술체육학
복합학
DBpia AI
AI 에이전트
AI 아이디어
AI 리더
AI 뷰어
Citeasy
크롬 서지관리 다운로드
지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록·키워드
Hyperthermia has recently been applied to treat human non-small cell lung cancer (NSCLC). However, the mechanisms underlying cytotoxic sensitivity of NSCLC cells to hyperthermia are not fully understood. In this study, five NSCLC cell lines with different epidermal growth factor receptor (EGFR), Kirsten rat sarcoma and tumor protein p53 mutation profiles (A549, H292, H1299, PC9 and H1975) were used to evaluate effects of hyperthermia. All tested cell lines except H1975 were sensitive to hyperthermia-induced cytotoxicity. Annexin V-propidium iodide double staining, Poly(ADP-ribose) polymerase (PARP) cleavage and scanning electron microscopy revealed that apoptosis and necrosis were induced by hyperthermia in different lines. Tetramethylrhodamine, ethyl ester analysis further revealed that hyperthermia affected mitochondrial function in the four hyperthermia-sensitive lines. Transmission electron microscopic analysis revealed degeneration of cristae and ruptured mitochondria upon exposure to hyperthermia. Hyperthermia also caused elevation of reactive oxygen species in sensitive cells. In addition to these effects, hyperthermia impacted cell survival-related signalling proteins (EGFR, FAK and Akt). In particular, hyperthermia increased phosphorylated EGFR but suppressed total EGFR, phosphorylated Akt and total Akt in sensitive cells. Moreover, hyperthermia could modulate immunomodulatory molecules. Major histocompatibility complex-I (MHC-I) and surface programmed death ligand-1 (PD-L1) were both elevated by hyperthermia in all tested NSCLC cell lines except PC9. Taken together, our findings provide insights into the potential influence of different somatic mutations in NSCLC cells on hyperthermia-induced cytotoxicity and regulation of key immunomodulatory molecules.
인공지능 문자 인식 모델을 통해 추출된 텍스트로, 일부 오타나 오류가 포함될 수 있으나 지속적으로 개선 중입니다.
오류를 발견하셨다면 해당 부분을 드래그한 후 ' 를 통해 신고해주세요.
오류를 발견하셨다면 해당 부분을 드래그한 후 ' 를 통해 신고해주세요.
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