인문학
사회과학
자연과학
공학
의약학
농수해양학
예술체육학
복합학
DBpia AI
AI 에이전트
AI 아이디어
AI 리더
AI 뷰어
Citeasy
크롬 서지관리 다운로드
지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록·키워드
Although plant viruses inflict billions of dollars in global crop yield loss, they also encode functions that can be used for crop protection. A viral vector attenuation strategy is developed that enables modulation of a 'gene therapy' viral vector designed for protective gene delivery. This attenuation vector targets its homologous native viral counterpart, where the A-component of the bipartite tomato mottle virus (ToMoV) viral vector delivers siRNA constructs targeting native viral sequences for which its own genome has been synthetically modified to avoid silencing. A target coat protein replacement viral vector was engineered with a Nano-luciferase reporter gene to quantify effectiveness based on bioluminescence. To silence the target virus, siRNA constructs were designed against the transactivating protein (TrAP) which is responsible for activating the promoter driving the native coat protein transcript. This siRNA was placed in the attenuation vector designed to avoid self-targeting by modifying its own TrAP sequence. Viral infections were initiated in tobacco using Agrobacterium DNA delivery. Measurements at 3, 6, and 9 days post infiltration revealed a significantly reduced luminescence in attenuation treatment demonstrating suppression of the target virus transgene expression. This work sets the stage for a breadth of virus attenuation studies that can be rapidly applied to diverse crop threats.
인공지능 문자 인식 모델을 통해 추출된 텍스트로, 일부 오타나 오류가 포함될 수 있으나 지속적으로 개선 중입니다.
오류를 발견하셨다면 해당 부분을 드래그한 후 ' 를 통해 신고해주세요.
오류를 발견하셨다면 해당 부분을 드래그한 후 ' 를 통해 신고해주세요.
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