인문학
사회과학
자연과학
공학
의약학
농수해양학
예술체육학
복합학
DBpia AI
AI 에이전트
AI 아이디어
AI 리더
AI 뷰어
Citeasy
크롬 서지관리 다운로드
지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록·키워드
ABSTRACT Microbial fermentation is a potent strategy for eco‐friendly and sustainable chitin oligosaccharide (CHOS) production. Nonetheless, hurdles (e.g., imbalanced metabolic flow and the need for uridine diphosphate (UDP)‐sugar donor consumption in CHOS synthesis) hinder enhanced and efficient production. In this study, we aimed to use Corynebacterium glutamicum as the foundational organism for de novo CHOS synthesis. Initially, we developed the CHOS synthesis pathway in C. glutamicum , attaining a CHOS titer of 113.34 mg/L. Furthermore, we fortified the uridine 5′‐diphospho‐ N ‐acetylglucosamine (UDP‐GlcNAc) synthesis module, vital for CHOS and other functional sugar synthesis, and developed a system for regenerating uridine triphosphate (UTP) precursors. Finally, we performed C. glutamicum ‐mediated scale‐up CHOS production in a 5‐L bioreactor yielding a titer of 5.08 g/L. The CHOS chassis strain provides a robust foundation for mass CHOS production via metabolic engineering. Altering the intracellular UDP‐sugar donor creation pathway could reportedly significantly enhance CHOS production. We attained the peak concentration of 829.33 mg/L with the heightened expression of glmM , glmU , and the metabolic equilibrium of PCM1 and AmgK . Bacterial growth remained unaffected by the excessive gene expressions or external gene incorporations. In addition, the swift growth and C. glutamicum accumulation in the fermenter led to increased CHOS production, reaching a titer of 5.08 g/L from the recombinant strain CGSL63, being 4.43 times higher than in the case of shake flask fermentation. The engineering strategies used in this study might be helpful for the C. glutamicum ‐mediated microbial synthesis of functional sugars. The methods applied in this study are broadly applicable for boosting the microbial generation of other valuable functional sugars.
인공지능 문자 인식 모델을 통해 추출된 텍스트로, 일부 오타나 오류가 포함될 수 있으나 지속적으로 개선 중입니다.
오류를 발견하셨다면 해당 부분을 드래그한 후 ' 를 통해 신고해주세요.
오류를 발견하셨다면 해당 부분을 드래그한 후 ' 를 통해 신고해주세요.
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