인문학
사회과학
자연과학
공학
의약학
농수해양학
예술체육학
복합학
DBpia AI
AI 에이전트
AI 아이디어
AI 리더
AI 뷰어
Citeasy
크롬 서지관리 다운로드
지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록·키워드
MicroRNAs (miRs) regulate the translation of target genes often in a cell-type specific manner. We previously demonstrated that downregulation of either miR-181a or miR-200c with intracranial injection of an inhibitor is protective against experimental stroke in mice. Here, we generated genetic lines of inducible Ca<sup>2+</sup>-calmodulin kinase IIα (CKIIα) neuronal miR-181a/b-1 and miR-141/200c cluster deletion to investigate whether the protective effect of their inhibition could be neuron-specific. Jackson Lab strains Mirc14tm1.1Czc/J and Mirc13tm1Mtm/Mmjax were each crossed with the tamoxifen-inducible Cre-recombinase strain B6;129S6-Tg, CKIIα-cre/ERT2. Adult double transgenic male mice were randomized and treated with 3 mg tamoxifen or vehicle via oral gavage for 7 days prior to 1 h middle cerebral artery occlusion (MCAO) or sham surgery. Mice were assessed for gross motor function at 24 h and then sacrificed for quantification of infarct volume. Separate animals were assessed for cell-type specific brain expression of miR-181a and miR-200c via combined fluorescent immunohistochemistry and in situ hybridization. Brains from tamoxifen treated mice exhibited selective miR deletion in CKIIα neurons. Infarct volumes were significantly lower, and neurological scores significantly improved in CKIIα/miR-200c mice pretreated with tamoxifen versus vehicle alone. In contrast, no difference was observed in infarct volume or neurological score in CKIIα/miR-181a mice pretreated with tamoxifen versus vehicle.
인공지능 문자 인식 모델을 통해 추출된 텍스트로, 일부 오타나 오류가 포함될 수 있으나 지속적으로 개선 중입니다.
오류를 발견하셨다면 해당 부분을 드래그한 후 ' 를 통해 신고해주세요.
오류를 발견하셨다면 해당 부분을 드래그한 후 ' 를 통해 신고해주세요.
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