인문학
사회과학
자연과학
공학
의약학
농수해양학
예술체육학
복합학
DBpia AI
AI 에이전트
AI 아이디어
AI 리더
AI 뷰어
Citeasy
크롬 서지관리 다운로드
지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록·키워드
Fruit color is a key quality trait in strawberry breeding and cultivar development, as it directly influences consumer preference and marketability. Anthocyanins are the pigments responsible for the red coloration in strawberries, and the transcription factor <i>MYB10</i> gene plays a crucial role in regulating the anthocyanin biosynthetic pathway. Our previous study identified a homoeolog-specific copy, <i>MYB10-1B</i>, located on chromosome 1B, as a key regulator of fruit color. The natural mutation in <i>MYB10-1B</i>, such as in the variety 'Florida Pearl' leads to the development of white fruit. Building on this discovery, we applied CRISPR/Cas9-mediated homoeolog-specific editing to target the functional dominant allele, <i>MYB10-1B</i>, in the cultivated octoploid strawberry 'Florida Brilliance', successfully altering the fruit color from red to white. Gene expression analysis in the edited lines revealed downregulation of <i>MYB10-1B</i> and key anthocyanin biosynthesis genes (<i>CHS</i>, <i>DFR</i>, and <i>ANS</i>). Furthermore, whole-genome resequencing results showed precise on-target mutations in <i>MYB10-1B</i> with minimal off-target effects. This study highlights the successful application of homoeolog-specific CRISPR/Cas9-mediated gene editing in polyploid species and provides a foundation for functional genomics and advanced breeding strategies in strawberries. Importantly, our findings demonstrate that specific targeting of the dominantly expressed homoeologous copy is essential for inducing phenotypic changes in polyploids. This underscores the importance of precise gene editing in octoploid strawberry to drive trait improvement.
인공지능 문자 인식 모델을 통해 추출된 텍스트로, 일부 오타나 오류가 포함될 수 있으나 지속적으로 개선 중입니다.
오류를 발견하셨다면 해당 부분을 드래그한 후 ' 를 통해 신고해주세요.
오류를 발견하셨다면 해당 부분을 드래그한 후 ' 를 통해 신고해주세요.
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