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Wiley MicrobiologyOpen 14(5)
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    초록·키워드

    Fish, including Oreochromis shiranus (Chambo), is a vital protein source in Malawi, but consumption has declined. Due to its perishability, preservation methods like smoking and frying are common. This study compares the microbial load in smoked and fried tilapia from wholesale and retail markets. This cross-sectional study compared the microbial load in smoked and fried tilapia (Chambo) from wholesale (Salima) and retail (Lilongwe) markets. Twenty fish samples (10 smoked and 10 fried) were collected, transported in sterile conditions, and analyzed for Total Aerobic Plate Count and coliforms using ISO-standard methods. Data was log-transformed and analyzed using two-way analysis of variance, with significance set at p < 0.05. Fried tilapia from wholesalers had low levels of contamination, with most samples showing too few colonies to count, while some smoked samples displayed higher bacterial counts (33.75 ± 3.77 CFU/mL). Retailer samples showed a wider range of bacterial species, including Escherichia coli and Pseudomonas putida, suggesting potential hygiene concerns. Overall, bacterial loads were significantly higher in tilapia from retail markets compared with those from wholesalers (p < 0.05). The analysis showed higher microbial contamination in tilapia from retailers, with greater bacterial diversity, including E. coli, indicating poor hygiene. Wholesaler samples had minimal contamination. Although preservation methods showed no significant difference in microbial loads, retailer fish had significantly higher bacterial loads.

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