인문학
사회과학
자연과학
공학
의약학
농수해양학
예술체육학
복합학
DBpia AI
AI 에이전트
AI 아이디어
AI 리더
AI 뷰어
Citeasy
크롬 서지관리 다운로드
지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록·키워드
Macrophages are considered the first line of defence against Mycoplasma pneumoniae infection in sheep, and their polarization significantly affects lung tissue damage and remodelling. Here, we collected sheep bronchoalveolar lavage fluid (BALF) 28 days after infection with Mycoplasma ovipneumoniae (MO). RNA-seq analysis revealed a significant increase in peptidyl arginine deiminase 2 (PADI2) expression in the MO group, and upregulated genes were significantly enriched in the "FoxO signalling pathway" and "AMPK signalling pathway". Immunohistofluorescence (IHF) staining revealed a significant increase in the number of macrophages, which were widely distributed in the alveolar cavity, in the MO infection group, and PADI2 was also positively expressed in the macrophages. In bone marrow-derived macrophages (BMDMs) isolated in vitro, inhibiting the expression of PADI2 induced infected macrophages to polarize towards alternatively activated (AAM, M2) macrophages. Moreover, the expression levels of the anti-inflammatory gene IL10 and tissue repair-related genes such as vascular endothelial growth factor alpha (VEGFA), transforming growth factor-β (TGF-β), and hepatocyte growth factor (HGF) significantly increased. In an in vitro coculture system, inhibition of PADI2 expression in BMDMs reduced apoptotic and intracellular reactive oxygen species (ROS) levels in lung fibroblasts. Interestingly, correlation analysis between the tracheal MO load and PADI2 expression in the bronchoalveolar lavage fluid of naturally infected MO sheep revealed that the higher the MO load was, the greater the PADI2 expression. A dual luciferase reporter assay confirmed that this process may be regulated by the transcription factor SP3. These results contribute to the understanding of the polarization state of macrophages after mycoplasma infection and to the preliminary exploration of PADI2 as an effective target to enhance host defence against MO infection. Targeting PADI2 to promote the polarization of M2 macrophages provides a promising approach for treating MO infection or preparing anti-Mycoplasma pneumonia gene-edited sheep.
인공지능 문자 인식 모델을 통해 추출된 텍스트로, 일부 오타나 오류가 포함될 수 있으나 지속적으로 개선 중입니다.
오류를 발견하셨다면 해당 부분을 드래그한 후 ' 를 통해 신고해주세요.
오류를 발견하셨다면 해당 부분을 드래그한 후 ' 를 통해 신고해주세요.
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