인문학
사회과학
자연과학
공학
의약학
농수해양학
예술체육학
복합학
DBpia AI
AI 에이전트
AI 아이디어
AI 리더
AI 뷰어
Citeasy
크롬 서지관리 다운로드
지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록·키워드
Abstract Background Natural antioxidants are crucial in slowing the progression of nervous system disorders such as multiple sclerosis (MS), primarily by counteracting oxidative stress and neuroinflammation-two central drivers of neuronal injury. Green tea (EGCG) and broccoli (sulforaphane, SF) extracts are rich in potent antioxidant and anti‑inflammatory compounds, yet their comparative neuroprotective efficacy against cuprizone (CPZ)‑induced toxicity has not been thoroughly investigated. Methods GC–MS confirmed catechins and caffeine as the main green tea constituents, and sulforaphane with flavonoids as predominant in broccoli. SH‑SY5Y neuroblastoma cells were exposed to CPZ (750 or 1000 µM) with or without extract pretreatment (EGCG, 5 or 10 µM; SF, 10 or 20 µM). Cell viability (MTT, trypan blue), membrane/nuclear morphology (light and fluorescence microscopy), and expression of MMP‑3, IL‑1β, NF‑κB1, HIF‑1α, and miR‑124‑3p were assessed. Results CPZ (750 µM) reduced viability by > 80% and induced marked structural damage. Optimal doses—EGCG 5 µM and SF 10 µM—significantly preserved cell viability and morphology. CPZ elevated MMP‑3 and suppressed NF‑κB1, HIF‑1α, and miR‑124‑3p, with no change in IL‑1β. EGCG alone markedly reduced MMP‑3 and IL‑1β; SF alone lowered IL‑1β and moderated miR‑124‑3p loss. SF + CPZ broadly attenuated CPZ effects, significantly reducing MMP‑3 and IL‑1β and restoring NF‑κB1 and HIF‑1α. EGCG + CPZ conferred selective protection but increased MMP‑3 relative to EGCG alone. Conclusion Both extracts mitigate CPZ‑induced neurotoxic signaling via distinct yet overlapping pathways—EGCG through targeted suppression of matrix degradation, SF through broader anti‑inflammatory and gene‑restorative effects. The current findings support their potential as complementary neuroprotective strategies, warranting further in vivo validation.
인공지능 문자 인식 모델을 통해 추출된 텍스트로, 일부 오타나 오류가 포함될 수 있으나 지속적으로 개선 중입니다.
오류를 발견하셨다면 해당 부분을 드래그한 후 ' 를 통해 신고해주세요.
오류를 발견하셨다면 해당 부분을 드래그한 후 ' 를 통해 신고해주세요.
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