인문학
사회과학
자연과학
공학
의약학
농수해양학
예술체육학
복합학
DBpia AI
AI 에이전트
AI 아이디어
AI 리더
AI 뷰어
Citeasy
크롬 서지관리 다운로드
지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록·키워드
Antibiotic resistance in Enterobacteriaceae continues to rise, and its implications for healthcare delivery have intensified. We investigated the genetic basis of antimicrobial resistance (AMR), virulence genes, and associated plasmids in Enterobacteriaceae isolates from a teaching hospital in Ghana. Antimicrobial susceptibility testing was performed using archived isolates. Whole genome sequencing was performed on a subset of the isolates, that were either multidrug resistant or extended-spectrum β-lactamase (ESBL)-producing. Bioinformatics analyses were performed for speciation, identification of AMR and virulence genes, and associated plasmids. The 100 Enterobacteriaceae isolates included in this study showed high phenotypic resistance to ß-lactams and susceptibility to aminoglycosides. Nineteen of the 20 WGS isolates were genotypically identified using the housekeeping genes as Escherichia coli (8/20, 40%), Klebsiella pneumoniae (8/20, 40%), Enterobacter cloacae (2/20, 10%), and Salmonella enterica (1/20, 5%). These strains harboured 139 unique antibiotic resistance genes (ARGs) encoding resistance against ß-lactams (64/139), aminoglycosides (23/139), fluoroquinolones (45/139), tetracyclines (37/139), phenicols (27/139), and sulphonamides (9/139). Subsequent AST revealed that (74/79, 94%) were ESBL producers, and (9/79,11%) were carbapenem resistant Enterobacteriaceae (CRE). The isolates expressed 8 categories of virulence factors (VFs), including effector delivery systems, adherence, and metabolic factors. Additionally, 26 unique plasmid replicons of both the I-complex and colicin plasmids were detected. We observed phenotypic and genotypic evidence of antimicrobial resistance to common antibiotics in the isolates driven by CTX-M-15 and in some K. pneumoniae, the NDM-1. These findings highlight the urgent need to improve antibiotic stewardship, surveillance and control at the hospital.
인공지능 문자 인식 모델을 통해 추출된 텍스트로, 일부 오타나 오류가 포함될 수 있으나 지속적으로 개선 중입니다.
오류를 발견하셨다면 해당 부분을 드래그한 후 ' 를 통해 신고해주세요.
오류를 발견하셨다면 해당 부분을 드래그한 후 ' 를 통해 신고해주세요.
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