인문학
사회과학
자연과학
공학
의약학
농수해양학
예술체육학
복합학
DBpia AI
AI 에이전트
AI 아이디어
AI 리더
AI 뷰어
Citeasy
크롬 서지관리 다운로드
지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록·키워드
Traumatic tracheal stenosis (TTS) is one of the most commonly seen iatrogenic airway injuries caused by tracheal intubation or tracheotomy, which can harm the lives of patients in severe cases. Circular RNA (circRNA) is related to a variety of human diseases. However, the role that circRNAs are likely to play in traumatic tracheal stenosis is not known to a large extent till now. This research is designed to identify potential biomarkers and learn more about the mechanism of TTS through the establishment of a circRNA-associated ceRNA network. By carrying out RNA sequencing (RNA-seq), the expression profiles of circRNAs and mRNAs were explored from four TTS patients and four normal control cases. To investigate the differentially expressed circRNAs and mRNAs, this research performed both Gene Ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses. A circRNA-miRNA-mRNA network was predicted and established using bioinformatics tools including Mireap, Miranda, TargetScan, the circBase database, and miRTarBase. And then verified by utilizing RT-qPCR assays from six TTS patients and six normal control cases. In clinical tissue samples of the TTS group and the control group, 603 circRNAs and 1950 mRNAs were differentially expressed. According to GO and KEGG analyses, the differentially expressed circRNAs mainly participated in lysine degradation, platelet activation, and the VEGF/HIF-1 signaling pathway, whereas the differentially expressed mRNAs mainly participated in cytokine-cytokine receptor interaction, ECM-receptor interaction, neutrophil extracellular trap formation, and the PI3K-Akt signaling pathway. By utilizing 17 circRNAs, 7 miRNAs, and 9 mRNAs, a competing endogenous RNA (ceRNA) network was set up. Using RT-qPCR assay, It was confirmed that S100A2 and circ_030284 were upregulated, while miR-1207-5p was downregulated in TTS patients compared to normal controls, consistent with the gene chip outcomes. The research presents a comprehensive circRNA-associated ceRNA network in patients with TTS, which provides novel insights into TTS’s underlying pathogenesis.
인공지능 문자 인식 모델을 통해 추출된 텍스트로, 일부 오타나 오류가 포함될 수 있으나 지속적으로 개선 중입니다.
오류를 발견하셨다면 해당 부분을 드래그한 후 ' 를 통해 신고해주세요.
오류를 발견하셨다면 해당 부분을 드래그한 후 ' 를 통해 신고해주세요.
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